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    Ultraviolet microbeam irradiations of mitotic diatoms: investigation of spindle elongation.

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    Author
    Leslie, RJ; Pickett-Heaps, JD
    Date
    1983-02
    Source Title
    The Journal of Cell Biology
    Publisher
    Rockefeller University Press
    University of Melbourne Author/s
    Pickett-Heaps, Jeremy
    Affiliation
    School of BioSciences
    Metadata
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    Document Type
    Journal Article
    Citations
    Leslie, R. J. & Pickett-Heaps, J. D. (1983). Ultraviolet microbeam irradiations of mitotic diatoms: investigation of spindle elongation.. J Cell Biol, 96 (2), pp.548-561. https://doi.org/10.1083/jcb.96.2.548.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/256732
    DOI
    10.1083/jcb.96.2.548
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112307
    Abstract
    Our simple instrumentation for generating a UV-microbeam is described UV microbeam irradiations of the central spindle in the pennate diatom Hantzschia amphioxys have been examined through correlated birefringence light microscopy and TEM. A precise correlation between the region of reduced birefringence and the UV-induced lesion in the microtubules (MTs) of the central spindle is demonstrated. The UV beam appears to dissociate MTs, as MT fragments were rarely encountered. The forces associated with metaphase and anaphase spindles have been studied via localized UV-microbeam irradiation of the central spindle. These spindles were found to be subjected to compressional forces, presumably exerted by stretched or contracting chromosomes. Comparisons are made with the results of other writers. These compressional forces caused the poles of a severed anaphase spindle to move toward each other and the center of the cell. As these poles moved centrally, the larger of the two postirradiational central spindle remnants elongated with a concomitant decrease in the length of the overlap. Metaphase spindles, in contrast, did not elongate nor lose their overlap region. Our interpretation is that the force for anaphase spindle elongation in Hantzschia is generated between half-spindles in the region of MT overlap.

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