De novo transcriptome assembly for the spiny mouse (Acomys cahirinus)
AuthorMamrot, J; Legaie, R; Ellery, SJ; Wilson, T; Seemann, T; Powell, DR; Gardner, DK; Walker, DW; Temple-Smith, P; Papenfuss, AT; ...
Source TitleScientific Reports
PublisherNATURE PUBLISHING GROUP
AffiliationSchool of BioSciences
Medical Biology (W.E.H.I.)
Microbiology and Immunology
Document TypeJournal Article
CitationsMamrot, J., Legaie, R., Ellery, S. J., Wilson, T., Seemann, T., Powell, D. R., Gardner, D. K., Walker, D. W., Temple-Smith, P., Papenfuss, A. T. & Dickinson, H. (2017). De novo transcriptome assembly for the spiny mouse (Acomys cahirinus). SCIENTIFIC REPORTS, 7 (1), https://doi.org/10.1038/s41598-017-09334-7.
Access StatusOpen Access
Spiny mice of the genus Acomys display several unique physiological traits, including menstruation and scar-free wound healing; characteristics that are exceedingly rare in mammals, and of considerable interest to the scientific community. These unique attributes, and the potential for spiny mice to accurately model human diseases, are driving increased use of this genus in biomedical research, however little genetic information is accessible for this species. This project aimed to generate a draft transcriptome for the Common spiny mouse (Acomys cahirinus). Illumina sequencing of RNA from 15 organ types (male and female) produced 451 million, 150 bp paired-end reads (92.4Gbp). An extensive survey of de novo transcriptome assembly approaches using Trinity, SOAPdenovo-Trans, and Oases at multiple kmer lengths was conducted, producing 50 single-kmer assemblies from this dataset. Non-redundant transcripts from all assemblies were merged into a meta-assembly using the EvidentialGene tr2aacds pipeline, producing the largest gene catalogue to date for Acomys cahirinus. This study provides the first detailed characterization of the spiny mouse transcriptome. It validates use of the EvidentialGene tr2aacds pipeline in mammals to augment conventional de novo assembly approaches, and provides a valuable scientific resource for further investigation into the unique physiological characteristics inherent in the genus Acomys.
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