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dc.contributor.authorUmeda, K
dc.contributor.authorZhao, J
dc.contributor.authorSimmons, P
dc.contributor.authorStanley, E
dc.contributor.authorElefanty, A
dc.contributor.authorNakayama, N
dc.date.accessioned2020-12-21T02:02:00Z
dc.date.available2020-12-21T02:02:00Z
dc.date.issued2012-06-13
dc.identifier.citationUmeda, K., Zhao, J., Simmons, P., Stanley, E., Elefanty, A. & Nakayama, N. (2012). Human chondrogenic paraxial mesoderm, directed specification and prospective isolation from pluripotent stem cells. SCIENTIFIC REPORTS, 2 (1), https://doi.org/10.1038/srep00455.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/256755
dc.description.abstractDirected specification and prospective isolation of chondrogenic paraxial mesoderm progeny from human pluripotent stem (PS) cells have not yet been achieved. Here we report the successful generation of KDR(-)PDGFRα(+) progeny expressing paraxial mesoderm genes and the mesendoderm reporter MIXL1-GFP in a chemically defined medium containing the canonical WNT signaling activator, BMP-inhibitor, and the Nodal/Activin/TGFβ signaling controller. Isolated (GFP(+))KDR(-)PDGFRα(+) mesoderm cells were sensitive to sequential addition of the three chondrogenic factors PDGF, TGFβ and BMP. Under these conditions, the cells showed robust chondrogenic activity in micromass culture, and generated a hyaline-like translucent cartilage particle in serum-free medium. In contrast, both STRO1(+) mesenchymal stem/stromal cells from adult human marrow and mesenchymal cells spontaneously arising from hPS cells showed a relatively weaker chondrogenic response in vitro, and formed more of the fibrotic cartilage particles. Thus, hPS cell-derived KDR(-)PDGFRα(+ )paraxial mesoderm-like cells have potential in engineered cartilage formation and cartilage repair.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.titleHuman chondrogenic paraxial mesoderm, directed specification and prospective isolation from pluripotent stem cells
dc.typeJournal Article
dc.identifier.doi10.1038/srep00455
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.source.titleScientific Reports
melbourne.source.volume2
melbourne.source.issue1
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1230693
melbourne.contributor.authorStanley, Edouard
melbourne.contributor.authorElefanty, Andrew
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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