CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells

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Skelton, RJP; Brady, B; Khoja, S; Sahoo, D; Engel, J; Arasaratnam, D; Saleh, KK; Abilez, OJ; Zhao, P; Stanley, EG; ...Date
2016-01-12Source Title
Stem Cell ReportsPublisher
CELL PRESSAffiliation
Paediatrics (RCH)Metadata
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Skelton, R. J. P., Brady, B., Khoja, S., Sahoo, D., Engel, J., Arasaratnam, D., Saleh, K. K., Abilez, O. J., Zhao, P., Stanley, E. G., Elefanty, A. G., Kwon, M., Elliott, D. A. & Ardehali, R. (2016). CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells. STEM CELL REPORTS, 6 (1), pp.95-108. https://doi.org/10.1016/j.stemcr.2015.11.006.Access Status
Open AccessAbstract
The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and endothelial cells in vivo. Collectively, our data show that CD13 and ROR2 identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. These markers represent valuable tools for further dissection of early human cardiac differentiation, and will enable a detailed assessment of human pluripotent stem cell-derived cardiac lineage cells for potential clinical applications.
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