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dc.contributor.authorSkelton, RJP
dc.contributor.authorBrady, B
dc.contributor.authorKhoja, S
dc.contributor.authorSahoo, D
dc.contributor.authorEngel, J
dc.contributor.authorArasaratnam, D
dc.contributor.authorSaleh, KK
dc.contributor.authorAbilez, OJ
dc.contributor.authorZhao, P
dc.contributor.authorStanley, EG
dc.contributor.authorElefanty, AG
dc.contributor.authorKwon, M
dc.contributor.authorElliott, DA
dc.contributor.authorArdehali, R
dc.date.accessioned2020-12-21T02:02:08Z
dc.date.available2020-12-21T02:02:08Z
dc.date.issued2016-01-12
dc.identifierpii: S2213-6711(15)00342-2
dc.identifier.citationSkelton, R. J. P., Brady, B., Khoja, S., Sahoo, D., Engel, J., Arasaratnam, D., Saleh, K. K., Abilez, O. J., Zhao, P., Stanley, E. G., Elefanty, A. G., Kwon, M., Elliott, D. A. & Ardehali, R. (2016). CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells. STEM CELL REPORTS, 6 (1), pp.95-108. https://doi.org/10.1016/j.stemcr.2015.11.006.
dc.identifier.issn2213-6711
dc.identifier.urihttp://hdl.handle.net/11343/256756
dc.description.abstractThe generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and endothelial cells in vivo. Collectively, our data show that CD13 and ROR2 identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. These markers represent valuable tools for further dissection of early human cardiac differentiation, and will enable a detailed assessment of human pluripotent stem cell-derived cardiac lineage cells for potential clinical applications.
dc.languageEnglish
dc.publisherCELL PRESS
dc.titleCD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
dc.typeJournal Article
dc.identifier.doi10.1016/j.stemcr.2015.11.006
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.source.titleStem Cell Reports
melbourne.source.volume6
melbourne.source.issue1
melbourne.source.pages95-108
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1230697
melbourne.contributor.authorStanley, Edouard
melbourne.contributor.authorElefanty, Andrew
melbourne.contributor.authorElliott, David
dc.identifier.eissn2213-6711
melbourne.accessrightsOpen Access


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