BASH: a tool for managing BeadArray spatial artefacts

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Author
Cairns, JM; Dunning, MJ; Ritchie, ME; Russell, R; Lynch, AGDate
2008-12-15Source Title
BioinformaticsPublisher
OXFORD UNIV PRESSUniversity of Melbourne Author/s
Ritchie, MatthewAffiliation
Medical Biology (W.E.H.I.)Metadata
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Journal ArticleCitations
Cairns, J. M., Dunning, M. J., Ritchie, M. E., Russell, R. & Lynch, A. G. (2008). BASH: a tool for managing BeadArray spatial artefacts. BIOINFORMATICS, 24 (24), pp.2921-2922. https://doi.org/10.1093/bioinformatics/btn557.Access Status
Open AccessAbstract
SUMMARY: With their many replicates and their random layouts, Illumina BeadArrays provide greater scope fordetecting spatial artefacts than do other microarray technologies. They are also robust to artefact exclusion, yet there is a lack of tools that can perform these tasks for Illumina. We present BASH, a tool for this purpose. BASH adopts the concepts of Harshlight, but implements them in a manner that utilizes the unique characteristics of the Illumina technology. Using bead-level data, spatial artefacts of various kinds can thus be identified and excluded from further analyses. AVAILABILITY: The beadarray Bioconductor package (version 1.10 onwards), www.bioconductor.org
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