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dc.contributor.authorLo, CY-W
dc.contributor.authorChen, S
dc.contributor.authorCreed, SJ
dc.contributor.authorKang, M
dc.contributor.authorZhao, N
dc.contributor.authorTang, BZ
dc.contributor.authorElgass, KD
dc.date.accessioned2020-12-21T02:08:32Z
dc.date.available2020-12-21T02:08:32Z
dc.date.issued2016-08-05
dc.identifierpii: srep30855
dc.identifier.citationLo, C. Y. -W., Chen, S., Creed, S. J., Kang, M., Zhao, N., Tang, B. Z. & Elgass, K. D. (2016). Novel super-resolution capable mitochondrial probe, MitoRed AIE, enables assessment of real-time molecular mitochondrial dynamics. Scientific Reports, 6 (1), https://doi.org/10.1038/srep30855.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/256801
dc.description.abstractMitochondria and mitochondrial dynamics play vital roles in health and disease. With the intricate nanometer-scale structure and rapid dynamics of mitochondria, super-resolution microscopy techniques possess great un-tapped potential to significantly contribute to understanding mitochondrial biology and kinetics. Here we present a novel mitochondrial probe (MitoRed AIE) suitable for live mitochondrial dynamics imaging and single particle tracking (SPT), together with a multi-dimensional data analysis approach to assess local mitochondrial (membrane) fluidity. The MitoRed AIE probe localizes primarily to mitochondrial membranes, with 95 ms fluorophore on-time delivering 106 photons/ms, characteristics which we exploit to demonstrate live cell 100 fps 3D time-lapse tracking of mitochondria. Combining our experimental and analytical approaches, we uncover mitochondrial dynamics at unprecedented time scales. This approach opens up a new regime into high spatio-temporal resolution dynamics in many areas of mitochondrial biology.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleNovel super-resolution capable mitochondrial probe, MitoRed AIE, enables assessment of real-time molecular mitochondrial dynamics
dc.typeJournal Article
dc.identifier.doi10.1038/srep30855
melbourne.affiliation.departmentSchool of Chemistry
melbourne.source.titleScientific Reports
melbourne.source.volume6
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1231948
melbourne.contributor.authorCHEN, SIJIE
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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