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dc.contributor.authorRaynaud, CM
dc.contributor.authorHalabi, N
dc.contributor.authorElliott, DA
dc.contributor.authorPasquier, J
dc.contributor.authorElefanty, AG
dc.contributor.authorStanley, EG
dc.contributor.authorRafii, A
dc.date.accessioned2020-12-21T02:20:46Z
dc.date.available2020-12-21T02:20:46Z
dc.date.issued2013-01-16
dc.identifierpii: PONE-D-12-35104
dc.identifier.citationRaynaud, C. M., Halabi, N., Elliott, D. A., Pasquier, J., Elefanty, A. G., Stanley, E. G. & Rafii, A. (2013). Human Embryonic Stem Cell Derived Mesenchymal Progenitors Express Cardiac Markers but Do Not Form Contractile Cardiomyocytes. PLOS ONE, 8 (1), https://doi.org/10.1371/journal.pone.0054524.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/256887
dc.description.abstractMesenchymal progenitors or stromal cells have shown promise as a therapeutic strategy for a range of diseases including heart failure. In this context, we explored the growth and differentiation potential of mesenchymal progenitors (MPs) derived in vitro from human embryonic stem cells (hESCs). Similar to MPs isolated from bone marrow, hESC derived MPs (hESC-MPs) efficiently differentiated into archetypical mesenchymal derivatives such as chondrocytes and adipocytes. Upon treatment with 5-Azacytidine or TGF-β1, hESC-MPs modified their morphology and up-regulated expression of key cardiac transcription factors such as NKX2-5, MEF2C, HAND2 and MYOCD. Nevertheless, NKX2-5+ hESC-MP derivatives did not form contractile cardiomyocytes, raising questions concerning the suitability of these cells as a platform for cardiomyocyte replacement therapy. Gene profiling experiments revealed that, although hESC-MP derived cells expressed a suite of cardiac related genes, they lacked the complete repertoire of genes associated with bona fide cardiomyocytes. Our results suggest that whilst agents such as TGF-β1 and 5-Azacytidine can induce expression of cardiac related genes, but treated cells retain a mesenchymal like phenotype.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleHuman Embryonic Stem Cell Derived Mesenchymal Progenitors Express Cardiac Markers but Do Not Form Contractile Cardiomyocytes
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0054524
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.source.titlePLoS One
melbourne.source.volume8
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1230673
melbourne.contributor.authorStanley, Edouard
melbourne.contributor.authorElefanty, Andrew
melbourne.contributor.authorHarvey, Adrienne
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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