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    Assessment of metabolic and mitochondrial dynamics in CD4+and CD8+T cells in virologically suppressed HIV-positive individuals on combination antiretroviral therapy

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    Author
    Masson, JJR; Murphy, AJ; Lee, MKS; Ostrowski, M; Crowe, SM; Palmer, CS
    Date
    2017-08-30
    Source Title
    PLoS One
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Palmer, Clovis
    Affiliation
    Microbiology and Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Masson, J. J. R., Murphy, A. J., Lee, M. K. S., Ostrowski, M., Crowe, S. M. & Palmer, C. S. (2017). Assessment of metabolic and mitochondrial dynamics in CD4+and CD8+T cells in virologically suppressed HIV-positive individuals on combination antiretroviral therapy. PLOS ONE, 12 (8), https://doi.org/10.1371/journal.pone.0183931.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/256934
    DOI
    10.1371/journal.pone.0183931
    Abstract
    Metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and mitochondrial biogenesis in subpopulations of CD4+ and CD8+ T cells from 18 virologically-suppressed HIV-positive individuals on combination antiretroviral therapy (cART; median CD4+ cell count: 728 cells/μl) and 13 HIV seronegative controls. Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production were also analysed in total CD4+ and CD8+ T cells. Among HIV+/cART individuals, expression of glucose transporter (Glut1) and mitochondrial density were highest within central memory and naïve CD4+ T cells, and lowest among effector memory and transitional memory T cells, with similar trends in HIV-negative controls. Compared to HIV-negative controls, there was a trend towards higher percentage of circulating CD4+Glut1+ T cells in HIV+/cART participants. There were no significant differences in mitochondrial dynamics between subject groups. Glut1 expression was positively correlated with mitochondrial density and MMP in total CD4+ T cells, while MMP was also positively correlated with ROS production in both CD4+ and CD8+ T cells. Our study characterizes specific metabolic features of CD4+ and CD8+ T cells in HIV-negative and HIV+/cART individuals and will invite future studies to explore the immunometabolic consequences of HIV infection.

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