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dc.contributor.authorMasson, JJR
dc.contributor.authorMurphy, AJ
dc.contributor.authorLee, MKS
dc.contributor.authorOstrowski, M
dc.contributor.authorCrowe, SM
dc.contributor.authorPalmer, CS
dc.date.accessioned2020-12-21T02:27:25Z
dc.date.available2020-12-21T02:27:25Z
dc.date.issued2017-08-30
dc.identifierpii: PONE-D-17-05227
dc.identifier.citationMasson, J. J. R., Murphy, A. J., Lee, M. K. S., Ostrowski, M., Crowe, S. M. & Palmer, C. S. (2017). Assessment of metabolic and mitochondrial dynamics in CD4+and CD8+T cells in virologically suppressed HIV-positive individuals on combination antiretroviral therapy. PLOS ONE, 12 (8), https://doi.org/10.1371/journal.pone.0183931.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/256934
dc.description.abstractMetabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and mitochondrial biogenesis in subpopulations of CD4+ and CD8+ T cells from 18 virologically-suppressed HIV-positive individuals on combination antiretroviral therapy (cART; median CD4+ cell count: 728 cells/μl) and 13 HIV seronegative controls. Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production were also analysed in total CD4+ and CD8+ T cells. Among HIV+/cART individuals, expression of glucose transporter (Glut1) and mitochondrial density were highest within central memory and naïve CD4+ T cells, and lowest among effector memory and transitional memory T cells, with similar trends in HIV-negative controls. Compared to HIV-negative controls, there was a trend towards higher percentage of circulating CD4+Glut1+ T cells in HIV+/cART participants. There were no significant differences in mitochondrial dynamics between subject groups. Glut1 expression was positively correlated with mitochondrial density and MMP in total CD4+ T cells, while MMP was also positively correlated with ROS production in both CD4+ and CD8+ T cells. Our study characterizes specific metabolic features of CD4+ and CD8+ T cells in HIV-negative and HIV+/cART individuals and will invite future studies to explore the immunometabolic consequences of HIV infection.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleAssessment of metabolic and mitochondrial dynamics in CD4+and CD8+T cells in virologically suppressed HIV-positive individuals on combination antiretroviral therapy
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0183931
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titlePLoS One
melbourne.source.volume12
melbourne.source.issue8
dc.rights.licenseCC BY
melbourne.elementsid1234764
melbourne.contributor.authorPalmer, Clovis
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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