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dc.contributor.authorYin, D
dc.contributor.authorRitchie, ME
dc.contributor.authorJabbari, JS
dc.contributor.authorBeck, T
dc.contributor.authorBlewitt, ME
dc.contributor.authorKeniry, A
dc.date.accessioned2020-12-21T03:03:50Z
dc.date.available2020-12-21T03:03:50Z
dc.date.issued2016-12-01
dc.identifierpii: S2213-5960(16)30130-1
dc.identifier.citationYin, D., Ritchie, M. E., Jabbari, J. S., Beck, T., Blewitt, M. E. & Keniry, A. (2016). High concordance between Illumina HiSeq2500 and NextSeq500 for reduced representation bisulfite sequencing (RRBS). GENOMICS DATA, 10, pp.97-100. https://doi.org/10.1016/j.gdata.2016.10.002.
dc.identifier.issn2213-5960
dc.identifier.urihttp://hdl.handle.net/11343/257056
dc.description.abstractReduced representation bisulfite sequencing (RRBS) provides an efficient method for measuring DNA methylation at single base resolution in regions of high CpG density. This technique has been extensively tested on the HiSeq2500, which uses a 4-colour detection method, however it is unclear if the method will also work on the NextSeq500 platform, which employs a 2-colour detection system. We created an RRBS library and sequenced it on both the HiSeq2500 and NextSeq500, and found no significant difference in the base composition of reads derived from either machine. Moreover, the methylation calls made from the data of each instrument were highly concordant, with methylation patterns across the genome appearing as expected. Therefore, RRBS can be sequenced on the Nextseq500 with comparable quality to that of the HiSeq2500. All sequencing data are deposited in the GEO database under accession number GSE87097.
dc.languageEnglish
dc.publisherELSEVIER SCIENCE BV
dc.titleHigh concordance between Illumina HiSeq2500 and NextSeq500 for reduced representation bisulfite sequencing (RRBS)
dc.typeJournal Article
dc.identifier.doi10.1016/j.gdata.2016.10.002
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleGenomics Data
melbourne.source.volume10
melbourne.source.pages97-100
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1113693
melbourne.contributor.authorBlewitt, Marnie
melbourne.contributor.authorRitchie, Matthew
melbourne.contributor.authorKeniry, Andrew
dc.identifier.eissn2213-5960
melbourne.accessrightsOpen Access


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