Show simple item record

dc.contributor.authorBuchert, M
dc.contributor.authorSchneider, S
dc.contributor.authorMeskenaite, V
dc.contributor.authorAdams, MT
dc.contributor.authorCanaani, E
dc.contributor.authorBaechi, T
dc.contributor.authorMoelling, K
dc.contributor.authorHovens, CM
dc.date.accessioned2020-12-21T03:08:31Z
dc.date.available2020-12-21T03:08:31Z
dc.date.issued1999-01-25
dc.identifier.citationBuchert, M., Schneider, S., Meskenaite, V., Adams, M. T., Canaani, E., Baechi, T., Moelling, K. & Hovens, C. M. (1999). The junction-associated protein AF-6 interacts and clusters with specific EPH receptor tyrosine kinases at specialized sites of cell-cell contact in the brain. JOURNAL OF CELL BIOLOGY, 144 (2), pp.361-371. https://doi.org/10.1083/jcb.144.2.361.
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/11343/257089
dc.description.abstractThe AF-6/afadin protein, which contains a single PDZ domain, forms a peripheral component of cell membranes at specialized sites of cell-cell junctions. To identify potential receptor-binding targets of AF-6 we screened the PDZ domain of AF-6 against a range of COOH-terminal peptides selected from receptors having potential PDZ domain-binding termini. The PDZ domain of AF-6 interacts with a subset of members of the Eph subfamily of RTKs via its COOH terminus both in vitro and in vivo. Cotransfection of a green fluorescent protein-tagged AF-6 fusion protein with full-length Eph receptors into heterologous cells induces a clustering of the Eph receptors and AF-6 at sites of cell-cell contact. Immunohistochemical analysis in the adult rat brain reveals coclustering of AF-6 with Eph receptors at postsynaptic membrane sites of excitatory synapses in the hippocampus. Furthermore, AF-6 is a substrate for a subgroup of Eph receptors and phosphorylation of AF-6 is dependent on a functional kinase domain of the receptor. The physical interaction of endogenous AF-6 with Eph receptors is demonstrated by coimmunoprecipitation from whole rat brain lysates. AF-6 is a candidate for mediating the clustering of Eph receptors at postsynaptic specializations in the adult rat brain.
dc.languageEnglish
dc.publisherROCKEFELLER UNIV PRESS
dc.titleThe junction-associated protein AF-6 interacts and clusters with specific EPH receptor tyrosine kinases at specialized sites of cell-cell contact in the brain
dc.typeJournal Article
dc.identifier.doi10.1083/jcb.144.2.361
melbourne.affiliation.departmentSurgery (RMH)
melbourne.source.titleThe Journal of Cell Biology
melbourne.source.volume144
melbourne.source.issue2
melbourne.source.pages361-371
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1242020
melbourne.contributor.authorHovens, Christopher
dc.identifier.eissn1540-8140
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record