University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Surgery (St Vincent's)
  • Surgery (St Vincent's) - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Surgery (St Vincent's)
  • Surgery (St Vincent's) - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    A fence barrier method of leading edge cell capture for explorative biochemical research.

    Thumbnail
    Download
    published version (782.8Kb)

    Citations
    Scopus
    Web of Science
    Altmetric
    1
    1
    Author
    Wager, LJ; Murray, RZ; Thompson, EW; Leavesley, DI
    Date
    2017-09-03
    Source Title
    Cell Adhesion and Migration
    Publisher
    Informa UK Limited
    University of Melbourne Author/s
    Thompson, Erik
    Affiliation
    Surgery (St Vincent's)
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Wager, L. J., Murray, R. Z., Thompson, E. W. & Leavesley, D. I. (2017). A fence barrier method of leading edge cell capture for explorative biochemical research.. Cell Adh Migr, 11 (5-6), pp.496-503. https://doi.org/10.1080/19336918.2016.1269997.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257129
    DOI
    10.1080/19336918.2016.1269997
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810785
    Abstract
    The scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue of choice. Here we present an adaptation that extends the utility of this model to encompass the collection of cells from the leading edge of migrating epithelial sheets making available explorative biochemical analyses. The method is scalable and does not require expensive apparatus, making it suitable for large and small laboratories alike. We detail the application of our method and exemplify proof of principle data derived from primary human keratinocyte cultures.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45770]
    • Surgery (St Vincent's) - Research Publications [324]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors