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dc.contributor.authorWager, LJ
dc.contributor.authorMurray, RZ
dc.contributor.authorThompson, EW
dc.contributor.authorLeavesley, DI
dc.date.accessioned2020-12-21T03:15:12Z
dc.date.available2020-12-21T03:15:12Z
dc.date.issued2017-09-03
dc.identifier.citationWager, L. J., Murray, R. Z., Thompson, E. W. & Leavesley, D. I. (2017). A fence barrier method of leading edge cell capture for explorative biochemical research.. Cell Adh Migr, 11 (5-6), pp.496-503. https://doi.org/10.1080/19336918.2016.1269997.
dc.identifier.issn1933-6918
dc.identifier.urihttp://hdl.handle.net/11343/257129
dc.description.abstractThe scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue of choice. Here we present an adaptation that extends the utility of this model to encompass the collection of cells from the leading edge of migrating epithelial sheets making available explorative biochemical analyses. The method is scalable and does not require expensive apparatus, making it suitable for large and small laboratories alike. We detail the application of our method and exemplify proof of principle data derived from primary human keratinocyte cultures.
dc.languageeng
dc.publisherInforma UK Limited
dc.titleA fence barrier method of leading edge cell capture for explorative biochemical research.
dc.typeJournal Article
dc.identifier.doi10.1080/19336918.2016.1269997
melbourne.affiliation.departmentSurgery (St Vincent's)
melbourne.source.titleCell Adhesion and Migration
melbourne.source.volume11
melbourne.source.issue5-6
melbourne.source.pages496-503
dc.rights.licenseCC BY
melbourne.elementsid1246298
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810785
melbourne.contributor.authorThompson, Erik
dc.identifier.eissn1933-6926
melbourne.accessrightsOpen Access


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