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dc.contributor.authorDoomun, SNE
dc.contributor.authorLoke, S
dc.contributor.authorO'Callaghan, S
dc.contributor.authorCallahan, DL
dc.date.accessioned2020-12-21T03:21:07Z
dc.date.available2020-12-21T03:21:07Z
dc.date.issued2016-12-01
dc.identifierpii: metabo6040042
dc.identifier.citationDoomun, S. N. E., Loke, S., O'Callaghan, S. & Callahan, D. L. (2016). A Simple Method for Measuring Carbon-13 Fatty Acid Enrichment in the Major Lipid Classes of Microalgae Using GC-MS. METABOLITES, 6 (4), https://doi.org/10.3390/metabo6040042.
dc.identifier.issn2218-1989
dc.identifier.urihttp://hdl.handle.net/11343/257167
dc.description.abstractA simple method for tracing carbon fixation and lipid synthesis in microalgae was developed using a combination of solid-phase extraction (SPE) and negative ion chemical ionisation gas chromatography mass spectrometry (NCI-GC-MS). NCI-GC-MS is an extremely sensitive technique that can produce an unfragmented molecular ion making this technique particularly useful for stable isotope enrichment studies. Derivatisation of fatty acids using pentafluorobenzyl bromide (PFBBr) allows the coupling of the high separation efficiency of GC and the measurement of unfragmented molecular ions for each of the fatty acids by single quadrupole MS. The key is that isotope spectra can be measured without interference from co-eluting fatty acids or other molecules. Pre-fractionation of lipid extracts by SPE allows the measurement of 13C isotope incorporation into the three main lipid classes (phospholipids, glycolipids, neutral lipids) in microalgae thus allowing the study of complex lipid biochemistry using relatively straightforward analytical technology. The high selectivity of GC is necessary as it allows the collection of mass spectra for individual fatty acids, including cis/trans isomers, of the PFB-derivatised fatty acids. The combination of solid-phase extraction and GC-MS enables the accurate determination of 13C incorporation into each lipid pool. Three solvent extraction protocols that are commonly used in lipidomics were also evaluated and are described here with regard to extraction efficiencies for lipid analysis in microalgae.
dc.languageEnglish
dc.publisherMDPI
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleA Simple Method for Measuring Carbon-13 Fatty Acid Enrichment in the Major Lipid Classes of Microalgae Using GC-MS
dc.typeJournal Article
dc.identifier.doi10.3390/metabo6040042
melbourne.affiliation.departmentSchool of BioSciences
melbourne.affiliation.departmentBio21
melbourne.source.titleMetabolites
melbourne.source.volume6
melbourne.source.issue4
dc.rights.licenseCC BY
melbourne.elementsid1117568
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192448
melbourne.contributor.authorO'Callaghan, Sean
melbourne.contributor.authorCALLAHAN, DAMIEN
melbourne.contributor.authorElahee Doomun, Sheik Nadeem
dc.identifier.eissn2218-1989
melbourne.accessrightsOpen Access


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