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dc.contributor.authorTessier, L
dc.contributor.authorCote, O
dc.contributor.authorClark, ME
dc.contributor.authorViel, L
dc.contributor.authorDiaz-Mendez, A
dc.contributor.authorAnders, S
dc.contributor.authorBienzle, D
dc.date.accessioned2020-12-21T03:22:12Z
dc.date.available2020-12-21T03:22:12Z
dc.date.issued2017-09-08
dc.identifierpii: 10.1186/s12864-017-4107-6
dc.identifier.citationTessier, L., Cote, O., Clark, M. E., Viel, L., Diaz-Mendez, A., Anders, S. & Bienzle, D. (2017). Impaired response of the bronchial epithelium to inflammation characterizes severe equine asthma. BMC GENOMICS, 18 (1), https://doi.org/10.1186/s12864-017-4107-6.
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/11343/257175
dc.description.abstractBACKGROUND: Severe equine asthma is a naturally occurring lung inflammatory disease of mature animals characterized by neutrophilic inflammation, bronchoconstriction, mucus hypersecretion and airway remodeling. Exacerbations are triggered by inhalation of dust and microbial components. Affected animals eventually are unable of aerobic performance. In this study transcriptomic differences between asthmatic and non-asthmatic animals in the response of the bronchial epithelium to an inhaled challenge were determined. RESULTS: Paired endobronchial biopsies were obtained pre- and post-challenge from asthmatic and non-asthmatic animals. The transcriptome, determined by RNA-seq and analyzed with edgeR, contained 111 genes differentially expressed (DE) after challenge between horses with and without asthma, and 81 of these were upregulated. Genes involved in neutrophil migration and activation were in central location in interaction networks, and related gene ontology terms were significantly overrepresented. Relative abundance of specific gene products as determined by immunohistochemistry was correlated with differential gene expression. Gene sets involved in neutrophil chemotaxis, immune and inflammatory response, secretion, blood coagulation and apoptosis were overrepresented among up-regulated genes, while the rhythmic process gene set was overrepresented among down-regulated genes. MMP1, IL8, TLR4 and MMP9 appeared to be the most important proteins in connecting the STRING protein network of DE genes. CONCLUSIONS: Several differentially expressed genes and networks in horses with asthma also contribute to human asthma, highlighting similarities between severe human adult and equine asthma. Neutrophil activation by the bronchial epithelium is suggested as the trigger of the inflammatory cascade in equine asthma, followed by epithelial injury and impaired repair and differentiation. Circadian rhythm dysregulation and the sonic Hedgehog pathway were identified as potential novel contributory factors in equine asthma.
dc.languageEnglish
dc.publisherBMC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleImpaired response of the bronchial epithelium to inflammation characterizes severe equine asthma
dc.typeJournal Article
dc.identifier.doi10.1186/s12864-017-4107-6
melbourne.affiliation.departmentVeterinary Biosciences
melbourne.source.titleBMC Genomics
melbourne.source.volume18
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1247904
melbourne.contributor.authorDiaz Mendez, Ricardo Andres
dc.identifier.eissn1471-2164
melbourne.accessrightsOpen Access


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