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dc.contributor.authorBraun, A
dc.contributor.authorDang, K
dc.contributor.authorBuslig, F
dc.contributor.authorBaird, MA
dc.contributor.authorDavidson, MW
dc.contributor.authorWaterman, CM
dc.contributor.authorMyers, KA
dc.date.accessioned2020-12-21T03:23:07Z
dc.date.available2020-12-21T03:23:07Z
dc.date.issued2014-07-07
dc.identifierpii: jcb.201401063
dc.identifier.citationBraun, A., Dang, K., Buslig, F., Baird, M. A., Davidson, M. W., Waterman, C. M. & Myers, K. A. (2014). Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells.. J Cell Biol, 206 (1), pp.97-112. https://doi.org/10.1083/jcb.201401063.
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/11343/257182
dc.description.abstractEndothelial cells (ECs) migrate directionally during angiogenesis and wound healing by polarizing to extracellular cues to guide directional movement. EC polarization is controlled by microtubule (MT) growth dynamics, which are regulated by MT-associated proteins (MAPs) that alter MT stability. Mitotic centromere-associated kinesin (MCAK) is a MAP that promotes MT disassembly within the mitotic spindle, yet its function in regulating MT dynamics to promote EC polarity and migration has not been investigated. We used high-resolution fluorescence microscopy coupled with computational image analysis to elucidate the role of MCAK in regulating MT growth dynamics, morphology, and directional migration of ECs. Our results show that MCAK-mediated depolymerization of MTs is specifically targeted to the trailing edge of polarized wound-edge ECs. Regulation of MCAK function is dependent on Aurora A kinase, which is regionally enhanced by signaling from the small guanosine triphosphatase, Rac1. Thus, a Rac1-Aurora A-MCAK signaling pathway mediates EC polarization and directional migration by promoting regional differences in MT dynamics in the leading and trailing cell edges.
dc.languageeng
dc.publisherRockefeller University Press
dc.titleRac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells.
dc.typeJournal Article
dc.identifier.doi10.1083/jcb.201401063
melbourne.affiliation.departmentMedicine and Radiology
melbourne.source.titleThe Journal of Cell Biology
melbourne.source.volume206
melbourne.source.issue1
melbourne.source.pages97-112
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1249268
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085700
melbourne.contributor.authorMyers, Kenneth
dc.identifier.eissn1540-8140
melbourne.accessrightsOpen Access


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