Kinesin-5 regulates the growth of the axon by acting as a brake on its microtubule array.
AuthorMyers, KA; Baas, PW
Source TitleThe Journal of Cell Biology
PublisherRockefeller University Press
University of Melbourne Author/sMyers, Kenneth
AffiliationMedicine and Radiology
Document TypeJournal Article
CitationsMyers, K. A. & Baas, P. W. (2007). Kinesin-5 regulates the growth of the axon by acting as a brake on its microtubule array.. J Cell Biol, 178 (6), pp.1081-1091. https://doi.org/10.1083/jcb.200702074.
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064629
Kinesin-5 is a homotetrameric motor protein that interacts with adjacent microtubules in the mitotic spindle. Kinesin-5 is also highly expressed in developing postmitotic neurons. Axons of cultured neurons experimentally depleted of kinesin-5 grow up to five times longer than controls and display more branches. The faster growth rates are accompanied by a doubling of the frequency of transport of short microtubules, suggesting a major role for kinesin-5 in the balance of motor-driven forces on the axonal microtubule array. Live-cell imaging reveals that the effects on axonal length of kinesin-5 depletion are caused partly by a lower propensity of the axon and newly forming branches to undergo bouts of retraction. Overexpression of wild-type kinesin-5, but not a rigor mutant of kinesin-5, has the inverse effect on axonal length. These results indicate that kinesin-5 imposes restrictions on the growth of the axon and does so at least in part by generating forces on the axonal microtubule array.
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