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dc.contributor.authorHe, Y
dc.contributor.authorFrancis, F
dc.contributor.authorMyers, KA
dc.contributor.authorYu, W
dc.contributor.authorBlack, MM
dc.contributor.authorBaas, PW
dc.date.accessioned2020-12-21T03:23:23Z
dc.date.available2020-12-21T03:23:23Z
dc.date.issued2005-02-28
dc.identifierpii: jcb.200407191
dc.identifier.citationHe, Y., Francis, F., Myers, K. A., Yu, W., Black, M. M. & Baas, P. W. (2005). Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments.. J Cell Biol, 168 (5), pp.697-703. https://doi.org/10.1083/jcb.200407191.
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/11343/257184
dc.description.abstractRecent studies have shown that the transport of microtubules (MTs) and neurofilaments (NFs) within the axon is rapid, infrequent, asynchronous, and bidirectional. Here, we used RNA interference to investigate the role of cytoplasmic dynein in powering these transport events. To reveal transport of MTs and NFs, we expressed EGFP-tagged tubulin or NF proteins in cultured rat sympathetic neurons and performed live-cell imaging of the fluorescent cytoskeletal elements in photobleached regions of the axon. The occurrence of anterograde MT and retrograde NF movements was significantly diminished in neurons that had been depleted of dynein heavy chain, whereas the occurrence of retrograde MT and anterograde NF movements was unaffected. These results support a cargo model for NF transport and a sliding filament model for MT transport.
dc.languageeng
dc.publisherRockefeller University Press
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titleRole of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments.
dc.typeJournal Article
dc.identifier.doi10.1083/jcb.200407191
melbourne.affiliation.departmentMedicine (Austin & Northern Health)
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleThe Journal of Cell Biology
melbourne.source.volume168
melbourne.source.issue5
melbourne.source.pages697-703
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1249398
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171826
melbourne.contributor.authorMyers, Kenneth
dc.identifier.eissn1540-8140
melbourne.accessrightsOpen Access


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