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dc.contributor.authorGabriele, L
dc.contributor.authorPhung, J
dc.contributor.authorFukumoto, J
dc.contributor.authorSegal, D
dc.contributor.authorWang, IM
dc.contributor.authorGiannakakou, P
dc.contributor.authorGiese, NA
dc.contributor.authorOzato, K
dc.contributor.authorMorse, HC
dc.date.accessioned2020-12-21T03:28:23Z
dc.date.available2020-12-21T03:28:23Z
dc.date.issued1999-08-02
dc.identifier.citationGabriele, L., Phung, J., Fukumoto, J., Segal, D., Wang, I. M., Giannakakou, P., Giese, N. A., Ozato, K. & Morse, H. C. (1999). Regulation of apoptosis in myeloid cells by interferon consensus sequence-binding protein.. J Exp Med, 190 (3), pp.411-421. https://doi.org/10.1084/jem.190.3.411.
dc.identifier.issn0022-1007
dc.identifier.urihttp://hdl.handle.net/11343/257219
dc.description.abstractMice with a null mutation of the gene encoding interferon consensus sequence-binding protein (ICSBP) develop a disease with marked expansion of granulocytes and macrophages that frequently progresses to a fatal blast crisis, thus resembling human chronic myelogenous leukemia (CML). One important feature of CML is decreased responsiveness of myeloid cells to apoptotic stimuli. Here we show that myeloid cells from mice deficient in ICSBP exhibit reduced spontaneous apoptosis and a significant decrease in sensitivity to apoptosis induced by DNA damage. In contrast, apoptosis in thymocytes from ICSBP-deficient mice is unaffected. We also show that overexpression of ICSBP in the human U937 monocytic cell line enhances the rate of spontaneous apoptosis and the sensitivity to apoptosis induced by etoposide, lipopolysaccharide plus ATP, or rapamycin. Programmed cell death induced by etoposide was specifically blocked by peptides inhibitory for the caspase-1 or caspase-3 subfamilies of caspases. Studies of proapoptotic genes showed that cells overexpressing ICSBP have enhanced expression of caspase-3 precursor protein. In addition, analyses of antiapoptotic genes showed that overexpression of ICSBP results in decreased expression of Bcl-X(L). These data suggest that ICSBP modulates survival of myeloid cells by regulating expression of apoptosis-related genes.
dc.languageeng
dc.publisherRockefeller University Press
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titleRegulation of apoptosis in myeloid cells by interferon consensus sequence-binding protein.
dc.typeJournal Article
dc.identifier.doi10.1084/jem.190.3.411
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleJournal of Experimental Medicine
melbourne.source.volume190
melbourne.source.issue3
melbourne.source.pages411-421
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1254473
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195590
melbourne.contributor.authorSegal, David
dc.identifier.eissn1540-9538
melbourne.accessrightsOpen Access


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