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dc.contributor.authorUshizawa, K
dc.contributor.authorHerath, CB
dc.contributor.authorKaneyama, K
dc.contributor.authorShiojima, S
dc.contributor.authorHirasawa, A
dc.contributor.authorTakahashi, T
dc.contributor.authorImai, K
dc.contributor.authorOchiai, K
dc.contributor.authorTokunaga, T
dc.contributor.authorTsunoda, Y
dc.contributor.authorTsujimoto, G
dc.contributor.authorHashizume, K
dc.date.accessioned2020-12-21T03:37:12Z
dc.date.available2020-12-21T03:37:12Z
dc.date.issued2004-11-24
dc.identifierpii: 1477-7827-2-77
dc.identifier.citationUshizawa, K., Herath, C. B., Kaneyama, K., Shiojima, S., Hirasawa, A., Takahashi, T., Imai, K., Ochiai, K., Tokunaga, T., Tsunoda, Y., Tsujimoto, G. & Hashizume, K. (2004). cDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period.. Reprod Biol Endocrinol, 2 (1), pp.77-. https://doi.org/10.1186/1477-7827-2-77.
dc.identifier.issn1477-7827
dc.identifier.urihttp://hdl.handle.net/11343/257282
dc.description.abstractBACKGROUND: After fertilization, embryo development involves differentiation, as well as development of the fetal body and extra-embryonic tissues until the moment of implantation. During this period various cellular and molecular changes take place with a genetic origin, e.g. the elongation of embryonic tissues, cell-cell contact between the mother and the embryo and placentation. To identify genetic profiles and search for new candidate molecules involved during this period, embryonic gene expression was analyzed with a custom designed utero-placental complementary DNA (cDNA) microarray. METHODS: Bovine embryos on days 7, 14 and 21, extra-embryonic membranes on day 28 and fetuses on days 28 were collected to represent early embryo, elongating embryo, pre-implantation embryo, post-implantation extra-embryonic membrane and fetus, respectively. Gene expression at these different time points was analyzed using our cDNA microarray. Two clustering algorithms such as k-means and hierarchical clustering methods identified the expression patterns of differentially expressed genes across pre-implantation period. Novel candidate genes were confirmed by real-time RT-PCR. RESULTS: In total, 1,773 individual genes were analyzed by complete k-means clustering. Comparison of day 7 and day 14 revealed most genes increased during this period, and a small number of genes exhibiting altered expression decreased as gestation progressed. Clustering analysis demonstrated that trophoblast-cell-specific molecules such as placental lactogens (PLs), prolactin-related proteins (PRPs), interferon-tau, and adhesion molecules apparently all play pivotal roles in the preparation needed for implantation, since their expression was remarkably enhanced during the pre-implantation period. The hierarchical clustering analysis and RT-PCR data revealed new functional roles for certain known genes (dickkopf-1, NPM, etc) as well as novel candidate genes (AW464053, AW465434, AW462349, AW485575) related to already established trophoblast-specific genes such as PLs and PRPs. CONCLUSIONS: A large number of genes in extra-embryonic membrane increased up to implantation and these profiles provide information fundamental to an understanding of extra-embryonic membrane differentiation and development. Genes in significant expression suggest novel molecules in trophoblast differentiation.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titlecDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period.
dc.typeJournal Article
dc.identifier.doi10.1186/1477-7827-2-77
melbourne.affiliation.departmentMedicine (Austin & Northern Health)
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleReproductive Biology and Endocrinology
melbourne.source.volume2
melbourne.source.issue1
melbourne.source.pages77-
dc.rights.licenseCC BY
melbourne.elementsid1255510
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC535809
melbourne.contributor.authorHerath, Chandana
dc.identifier.eissn1477-7827
melbourne.accessrightsOpen Access


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