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dc.contributor.authorFrost, HR
dc.contributor.authorLaho, D
dc.contributor.authorSanderson-Smith, ML
dc.contributor.authorLicciardi, P
dc.contributor.authorDonath, S
dc.contributor.authorCurtis, N
dc.contributor.authorKado, J
dc.contributor.authorDale, JB
dc.contributor.authorSteer, AC
dc.contributor.authorSmeesters, PR
dc.date.accessioned2020-12-21T03:37:29Z
dc.date.available2020-12-21T03:37:29Z
dc.date.issued2017-11-01
dc.identifierpii: 3979656
dc.identifier.citationFrost, H. R., Laho, D., Sanderson-Smith, M. L., Licciardi, P., Donath, S., Curtis, N., Kado, J., Dale, J. B., Steer, A. C. & Smeesters, P. R. (2017). Immune Cross-Opsonization Within emm Clusters Following Group A Streptococcus Skin Infection: Broadening the Scope of Type-Specific Immunity. CLINICAL INFECTIOUS DISEASES, 65 (9), pp.1523-1531. https://doi.org/10.1093/cid/cix599.
dc.identifier.issn1058-4838
dc.identifier.urihttp://hdl.handle.net/11343/257284
dc.description.abstractBackground: Group A Streptococcus (GAS) skin infections are particularly prevalent in developing nations. The GAS M protein, by which strains are differentiated into >220 different emm types, is immunogenic and elicits protective antibodies. A major obstacle for vaccine development has been the traditional understanding that immunity following infection is restricted to a single emm type. However, recent evidence has led to the hypothesis of immune cross-reactivity between emm types. Methods: We investigated the human serological response to GAS impetigo in Fijian schoolchildren, focusing on 3 major emm clusters (E4, E6, and D4). Pre- and postinfection sera were assayed by enzyme-linked immunosorbent assay with N-terminal M peptides and bactericidal assays using the infecting-type strain, emm cluster-related strains, and nonrelated strains. Results: Twenty of the 53 paired sera demonstrated a ≥4-fold increase in antibody titer against the infecting type. When tested against all cluster-related M peptides, we found that 9 of 17 (53%) paired sera had a ≥4-fold increase in antibody titer to cluster-related strains as well. When grouped by cluster, the mean change to cluster-related emm types in E4 and E6 was >4-fold (5.9-fold and 19.5-fold, respectively) but for D4 was 3.8-fold. The 17 paired sera were tested in bactericidal assays against selected cluster-related and nonrelated strains. While the responses were highly variable, numerous instances of cross-reactive killing were observed. Conclusions: These data demonstrate that M type-specific and cross-reactive immune responses occur following skin infection. The cross-reactive immune responses frequently align with emm clusters, raising new opportunities to design multivalent vaccines with broad coverage.
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS INC
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0
dc.titleImmune Cross-Opsonization Within emm Clusters Following Group A Streptococcus Skin Infection: Broadening the Scope of Type-Specific Immunity
dc.typeJournal Article
dc.identifier.doi10.1093/cid/cix599
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.source.titleClinical Infectious Diseases
melbourne.source.volume65
melbourne.source.issue9
melbourne.source.pages1523-1531
dc.rights.licenseCC BY-NC
melbourne.elementsid1255721
melbourne.contributor.authorCurtis, Richard
melbourne.contributor.authorDonath, Susan
melbourne.contributor.authorSteer, Andrew
melbourne.contributor.authorSmeesters, Pierre
melbourne.contributor.authorLicciardi, Paul
dc.identifier.eissn1537-6591
melbourne.accessrightsOpen Access


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