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dc.contributor.authorWojdacz, TK
dc.contributor.authorHansen, LL
dc.contributor.authorDobrovic, A
dc.date.accessioned2020-12-21T03:38:29Z
dc.date.available2020-12-21T03:38:29Z
dc.date.issued2008-07-28
dc.identifierpii: 1756-0500-1-54
dc.identifier.citationWojdacz, T. K., Hansen, L. L. & Dobrovic, A. (2008). A new approach to primer design for the control of PCR bias in methylation studies.. BMC Res Notes, 1 (1), pp.54-. https://doi.org/10.1186/1756-0500-1-54.
dc.identifier.issn1756-0500
dc.identifier.urihttp://hdl.handle.net/11343/257291
dc.description.abstractPrimer design for PCR-based methylation analysis following bisulfite conversion of DNA is considerably more complex than primer design for regular PCR. The choice of the optimal primer set is critical to the performance and correct interpretation of the results. Most methodologies in methylation analysis utilize primers that theoretically amplify methylated and unmethylated templates at the same time. The proportional amplification of all templates is critical but difficult to achieve due to PCR bias favouring the amplification of the unmethylated template. The focus of this brief communication is to point out the important criteria needed for the successful choice of primers that will enable the control of PCR bias in bisulfite based methylation-screening protocols.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleA new approach to primer design for the control of PCR bias in methylation studies.
dc.typeJournal Article
dc.identifier.doi10.1186/1756-0500-1-54
melbourne.affiliation.departmentSurgery (Austin & Northern Health)
melbourne.source.titleBMC Research Notes
melbourne.source.volume1
melbourne.source.issue1
melbourne.source.pages54-
dc.rights.licenseCC BY
melbourne.elementsid1260054
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2525644
melbourne.contributor.authorDobrovic, Alexander
dc.identifier.eissn1756-0500
melbourne.accessrightsOpen Access


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