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    Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity

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    Author
    Nelson, AW; Groen, AJ; Miller, JL; Warren, AY; Holmes, KA; Tarulli, GA; Tilley, WD; Katzenellenbogen, BS; Hawse, JR; Gnanapragasam, VJ; ...
    Date
    2017-01-15
    Source Title
    Molecular and Cellular Endocrinology
    Publisher
    ELSEVIER IRELAND LTD
    University of Melbourne Author/s
    Tarulli, Gerard
    Affiliation
    School of BioSciences
    Metadata
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    Document Type
    Journal Article
    Citations
    Nelson, A. W., Groen, A. J., Miller, J. L., Warren, A. Y., Holmes, K. A., Tarulli, G. A., Tilley, W. D., Katzenellenbogen, B. S., Hawse, J. R., Gnanapragasam, V. J. & Carroll, J. S. (2017). Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 440 (C), pp.138-150. https://doi.org/10.1016/j.mce.2016.11.016.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257340
    DOI
    10.1016/j.mce.2016.11.016
    Abstract
    Estrogen Receptor-β (ERβ) has been implicated in many cancers. In prostate and breast cancer its function is controversial, but genetic studies implicate a role in cancer progression. Much of the confusion around ERβ stems from antibodies that are inadequately validated, yet have become standard tools for deciphering its role. Using an ERβ-inducible cell system we assessed commonly utilized ERβ antibodies and show that one of the most commonly used antibodies, NCL-ER-BETA, is non-specific for ERβ. Other antibodies have limited ERβ specificity or are only specific in one experimental modality. ERβ is commonly studied in MCF-7 (breast) and LNCaP (prostate) cancer cell lines, but we found no ERβ expression in either, using validated antibodies and independent mass spectrometry-based approaches. Our findings question conclusions made about ERβ using the NCL-ER-BETA antibody, or LNCaP and MCF-7 cell lines. We describe robust reagents, which detect ERβ across multiple experimental approaches and in clinical samples.

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