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dc.contributor.authorKoepfli, C
dc.contributor.authorNguitragool, W
dc.contributor.authorHofmann, NE
dc.contributor.authorRobinson, LJ
dc.contributor.authorOme-Kaius, M
dc.contributor.authorSattabongkot, J
dc.contributor.authorFelger, I
dc.contributor.authorMueller, I
dc.date.accessioned2020-12-21T03:49:11Z
dc.date.available2020-12-21T03:49:11Z
dc.date.issued2016-12-16
dc.identifierpii: srep39183
dc.identifier.citationKoepfli, C., Nguitragool, W., Hofmann, N. E., Robinson, L. J., Ome-Kaius, M., Sattabongkot, J., Felger, I. & Mueller, I. (2016). Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR). SCIENTIFIC REPORTS, 6 (1), https://doi.org/10.1038/srep39183.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/257366
dc.description.abstractAccurate quantification of parasite density in the human host is essential for understanding the biology and pathology of malaria. Semi-quantitative molecular methods are widely applied, but the need for an external standard curve makes it difficult to compare parasite density estimates across studies. Droplet digital PCR (ddPCR) allows direct quantification without the need for a standard curve. ddPCR was used to diagnose and quantify P. falciparum and P. vivax in clinical patients as well as in asymptomatic samples. ddPCR yielded highly reproducible measurements across the range of parasite densities observed in humans, and showed higher sensitivity than qPCR to diagnose P. falciparum, and equal sensitivity for P. vivax. Correspondence in quantification was very high (>0.95) between qPCR and ddPCR. Quantification between technical replicates by ddPCR differed 1.5-1.7-fold, compared to 2.4-6.2-fold by qPCR. ddPCR facilitates parasite quantification for studies where absolute densities are required, and will increase comparability of results reported from different laboratories.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleSensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)
dc.typeJournal Article
dc.identifier.doi10.1038/srep39183
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleScientific Reports
melbourne.source.volume6
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1122539
melbourne.contributor.authorKoepfli, Cristian
melbourne.contributor.authorRobinson, Leanne
melbourne.contributor.authorMueller, Ivo
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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