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    Fast X-Ray Fluorescence Microtomography of Hydrated Biological Samples

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    Author
    Lombi, E; de Jonge, MD; Donner, E; Kopittke, PM; Howard, DL; Kirkham, R; Ryan, CG; Paterson, D
    Date
    2011-06-02
    Source Title
    PLoS One
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Ryan, Christopher
    Affiliation
    School of Physics
    Metadata
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    Document Type
    Journal Article
    Citations
    Lombi, E., de Jonge, M. D., Donner, E., Kopittke, P. M., Howard, D. L., Kirkham, R., Ryan, C. G. & Paterson, D. (2011). Fast X-Ray Fluorescence Microtomography of Hydrated Biological Samples. PLOS ONE, 6 (6), https://doi.org/10.1371/journal.pone.0020626.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257458
    DOI
    10.1371/journal.pone.0020626
    Abstract
    Metals and metalloids play a key role in plant and other biological systems as some of them are essential to living organisms and all can be toxic at high concentrations. It is therefore important to understand how they are accumulated, complexed and transported within plants. In situ imaging of metal distribution at physiological relevant concentrations in highly hydrated biological systems is technically challenging. In the case of roots, this is mainly due to the possibility of artifacts arising during sample preparation such as cross sectioning. Synchrotron x-ray fluorescence microtomography has been used to obtain virtual cross sections of elemental distributions. However, traditionally this technique requires long data acquisition times. This has prohibited its application to highly hydrated biological samples which suffer both radiation damage and dehydration during extended analysis. However, recent advances in fast detectors coupled with powerful data acquisition approaches and suitable sample preparation methods can circumvent this problem. We demonstrate the heightened potential of this technique by imaging the distribution of nickel and zinc in hydrated plant roots. Although 3D tomography was still impeded by radiation damage, we successfully collected 2D tomograms of hydrated plant roots exposed to environmentally relevant metal concentrations for short periods of time. To our knowledge, this is the first published example of the possibilities offered by a new generation of fast fluorescence detectors to investigate metal and metalloid distribution in radiation-sensitive, biological samples.

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