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    Constraints on lateral gene transfer in promoting fimbrial usher protein diversity and function

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    Author
    Stubenrauch, CJ; Dougan, G; Lithgow, T; Heinz, E
    Date
    2017-11-01
    Source Title
    Open Biology
    Publisher
    ROYAL SOC
    University of Melbourne Author/s
    Dougan, Gordon
    Affiliation
    Microbiology and Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Stubenrauch, C. J., Dougan, G., Lithgow, T. & Heinz, E. (2017). Constraints on lateral gene transfer in promoting fimbrial usher protein diversity and function. OPEN BIOLOGY, 7 (11), https://doi.org/10.1098/rsob.170144.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257473
    DOI
    10.1098/rsob.170144
    Abstract
    Fimbriae are long, adhesive structures widespread throughout members of the family Enterobacteriaceae. They are multimeric extrusions, which are moved out of the bacterial cell through an integral outer membrane protein called usher. The complex folding mechanics of the usher protein were recently revealed to be catalysed by the membrane-embedded translocation and assembly module (TAM). Here, we examine the diversity of usher proteins across a wide range of extraintestinal (ExPEC) and enteropathogenic (EPEC) Escherichia coli, and further focus on a so far undescribed chaperone-usher system, with this usher referred to as UshC. The fimbrial system containing UshC is distributed across a discrete set of EPEC types, including model strains like E2348/67, as well as ExPEC ST131, currently the most prominent multi-drug-resistant uropathogenic E. coli strain worldwide. Deletion of the TAM from a naive strain of E. coli results in a drastic time delay in folding of UshC, which can be observed for a protein from EPEC as well as for two introduced proteins from related organisms, Yersinia and Enterobacter We suggest that this models why the TAM machinery is essential for efficient folding of proteins acquired via lateral gene transfer.

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