HPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples
AuthorCornall, AM; Poljak, M; Garland, SM; Phillips, S; Machalek, DA; Tan, JH; Quinn, MA; Tabrizi, SN
Source TitlePapillomavirus Research
PublisherELSEVIER SCIENCE BV
University of Melbourne Author/sMachalek, Dorothy; Cornall, Alyssa; Wark, Suzanne; Tan, Jeffrey; Tabrizi, Sepehr; QUINN, MICHAEL
AffiliationObstetrics and Gynaecology
Melbourne School of Population and Global Health
Document TypeJournal Article
CitationsCornall, A. M., Poljak, M., Garland, S. M., Phillips, S., Machalek, D. A., Tan, J. H., Quinn, M. A. & Tabrizi, S. N. (2017). HPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples. PAPILLOMAVIRUS RESEARCH, 4, pp.79-84. https://doi.org/10.1016/j.pvr.2017.10.002.
Access StatusOpen Access
PURPOSE: To compare human papillomavirus genotype-specific performance of two genotyping assays, Anyplex II HPV28 (Seegene) and EuroArray HPV (EuroImmun), with Linear Array HPV (Roche). METHODS: DNA extracted from clinican-collected cervical brush specimens in PreservCyt medium (Hologic), from 403 women undergoing management for detected cytological abnormalities, was tested on the three assays. Genotype-specific agreement were assessed by Cohen's kappa statistic and Fisher's z-test of significance between proportions. RESULTS: Agreement between Linear Array and the other 2 assays was substantial to almost perfect (κ = 0.60 - 1.00) for most genotypes, and was almost perfect (κ = 0.81 - 0.98) for almost all high-risk genotypes. Linear Array overall detected most genotypes more frequently, however this was only statistically significant for HPV51 (EuroArray; p = 0.0497), HPV52 (Anyplex II; p = 0.039) and HPV61 (Anyplex II; p=0.047). EuroArray detected signficantly more HPV26 (p = 0.002) and Anyplex II detected more HPV42 (p = 0.035) than Linear Array. Each assay performed differently for HPV68 detection: EuroArray and LA were in moderate to substantial agreement with Anyplex II (κ = 0.46 and 0.62, respectively), but were in poor disagreement with each other (κ = -0.01). CONCLUSIONS: EuroArray and Anyplex II had similar sensitivity to Linear Array for most high-risk genotypes, with slightly lower sensitivity for HPV 51 or 52.
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