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dc.contributor.authorCornall, AM
dc.contributor.authorPoljak, M
dc.contributor.authorGarland, SM
dc.contributor.authorPhillips, S
dc.contributor.authorMachalek, DA
dc.contributor.authorTan, JH
dc.contributor.authorQuinn, MA
dc.contributor.authorTabrizi, SN
dc.date.accessioned2020-12-21T04:08:07Z
dc.date.available2020-12-21T04:08:07Z
dc.date.issued2017-12-01
dc.identifierpii: S2405-8521(17)30009-5
dc.identifier.citationCornall, A. M., Poljak, M., Garland, S. M., Phillips, S., Machalek, D. A., Tan, J. H., Quinn, M. A. & Tabrizi, S. N. (2017). HPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples. PAPILLOMAVIRUS RESEARCH, 4, pp.79-84. https://doi.org/10.1016/j.pvr.2017.10.002.
dc.identifier.issn2405-8521
dc.identifier.urihttp://hdl.handle.net/11343/257496
dc.description.abstractPURPOSE: To compare human papillomavirus genotype-specific performance of two genotyping assays, Anyplex II HPV28 (Seegene) and EuroArray HPV (EuroImmun), with Linear Array HPV (Roche). METHODS: DNA extracted from clinican-collected cervical brush specimens in PreservCyt medium (Hologic), from 403 women undergoing management for detected cytological abnormalities, was tested on the three assays. Genotype-specific agreement were assessed by Cohen's kappa statistic and Fisher's z-test of significance between proportions. RESULTS: Agreement between Linear Array and the other 2 assays was substantial to almost perfect (κ = 0.60 - 1.00) for most genotypes, and was almost perfect (κ = 0.81 - 0.98) for almost all high-risk genotypes. Linear Array overall detected most genotypes more frequently, however this was only statistically significant for HPV51 (EuroArray; p = 0.0497), HPV52 (Anyplex II; p = 0.039) and HPV61 (Anyplex II; p=0.047). EuroArray detected signficantly more HPV26 (p = 0.002) and Anyplex II detected more HPV42 (p = 0.035) than Linear Array. Each assay performed differently for HPV68 detection: EuroArray and LA were in moderate to substantial agreement with Anyplex II (κ = 0.46 and 0.62, respectively), but were in poor disagreement with each other (κ = -0.01). CONCLUSIONS: EuroArray and Anyplex II had similar sensitivity to Linear Array for most high-risk genotypes, with slightly lower sensitivity for HPV 51 or 52.
dc.languageEnglish
dc.publisherELSEVIER SCIENCE BV
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.titleHPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples
dc.typeJournal Article
dc.identifier.doi10.1016/j.pvr.2017.10.002
melbourne.affiliation.departmentObstetrics and Gynaecology
melbourne.affiliation.departmentMelbourne School of Population and Global Health
melbourne.source.titlePapillomavirus Research
melbourne.source.volume4
melbourne.source.pages79-84
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1270770
melbourne.contributor.authorMachalek, Dorothy
melbourne.contributor.authorCornall, Alyssa
melbourne.contributor.authorWark, Suzanne
melbourne.contributor.authorTan, Jeffrey
melbourne.contributor.authorTabrizi, Sepehr
melbourne.contributor.authorQUINN, MICHAEL
dc.identifier.eissn2405-8521
melbourne.accessrightsOpen Access


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