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dc.contributor.authorLaprell, L
dc.contributor.authorRepak, E
dc.contributor.authorFranckevicius, V
dc.contributor.authorHartrampf, F
dc.contributor.authorTerhag, J
dc.contributor.authorHollmann, M
dc.contributor.authorSumser, M
dc.contributor.authorRebola, N
dc.contributor.authorDiGregorio, DA
dc.contributor.authorTrauner, D
dc.date.accessioned2020-12-21T04:14:43Z
dc.date.available2020-12-21T04:14:43Z
dc.date.issued2015-08-01
dc.identifierpii: ncomms9076
dc.identifier.citationLaprell, L., Repak, E., Franckevicius, V., Hartrampf, F., Terhag, J., Hollmann, M., Sumser, M., Rebola, N., DiGregorio, D. A. & Trauner, D. (2015). Optical control of NMDA receptors with a diffusible photoswitch. NATURE COMMUNICATIONS, 6 (1), https://doi.org/10.1038/ncomms9076.
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/11343/257543
dc.description.abstractN-methyl-D-aspartate receptors (NMDARs) play a central role in synaptic plasticity, learning and memory, and are implicated in various neuronal disorders. We synthesized a diffusible photochromic glutamate analogue, azobenzene-triazole-glutamate (ATG), which is specific for NMDARs and functions as a photoswitchable agonist. ATG is inactive in its dark-adapted trans-isoform, but can be converted into its active cis-isoform using one-photon (near UV) or two-photon (740 nm) excitation. Irradiation with violet light photo-inactivates ATG within milliseconds, allowing agonist removal on the timescale of NMDAR deactivation. ATG is compatible with Ca(2+) imaging and can be used to optically mimic synaptic coincidence detection protocols. Thus, ATG can be used like traditional caged glutamate compounds, but with the added advantages of NMDAR specificity, low antagonism of GABAR-mediated currents, and precise temporal control of agonist delivery.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.titleOptical control of NMDA receptors with a diffusible photoswitch
dc.typeJournal Article
dc.identifier.doi10.1038/ncomms9076
melbourne.affiliation.departmentFlorey Department of Neuroscience and Mental Health
melbourne.source.titleNature Communications
melbourne.source.volume6
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1177606
melbourne.contributor.authorTerhag, Jan
dc.identifier.eissn2041-1723
melbourne.accessrightsOpen Access


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