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    Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP

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    Author
    Wickramasinghe, VO; Andrews, R; Ellis, P; Langford, C; Gurdon, JB; Stewart, M; Venkitaraman, AR; Laskey, RA
    Date
    2014-04-01
    Source Title
    Nucleic Acids Research
    Publisher
    OXFORD UNIV PRESS
    University of Melbourne Author/s
    Wickramasinghe, Vihandha
    Affiliation
    Biochemistry and Molecular Biology
    Metadata
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    Document Type
    Journal Article
    Citations
    Wickramasinghe, V. O., Andrews, R., Ellis, P., Langford, C., Gurdon, J. B., Stewart, M., Venkitaraman, A. R. & Laskey, R. A. (2014). Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP. NUCLEIC ACIDS RESEARCH, 42 (8), pp.5059-5071. https://doi.org/10.1093/nar/gku095.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257550
    DOI
    10.1093/nar/gku095
    Abstract
    The nuclear phase of the gene expression pathway culminates in the export of mature messenger RNAs (mRNAs) to the cytoplasm through nuclear pore complexes. GANP (germinal- centre associated nuclear protein) promotes the transfer of mRNAs bound to the transport factor NXF1 to nuclear pore complexes. Here, we demonstrate that GANP, subunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent transcripts were highly expressed, yet short-lived, and were highly enriched in those encoding central components of the gene expression machinery such as RNA synthesis and processing factors. After injection into Xenopus oocyte nuclei, representative GANP-dependent transcripts showed faster nuclear export kinetics than representative transcripts that were not influenced by GANP depletion. We propose that GANP promotes the nuclear export of specific classes of mRNAs that may facilitate rapid changes in gene expression.

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