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dc.contributor.authorWickramasinghe, VO
dc.contributor.authorAndrews, R
dc.contributor.authorEllis, P
dc.contributor.authorLangford, C
dc.contributor.authorGurdon, JB
dc.contributor.authorStewart, M
dc.contributor.authorVenkitaraman, AR
dc.contributor.authorLaskey, RA
dc.date.accessioned2020-12-21T04:16:01Z
dc.date.available2020-12-21T04:16:01Z
dc.date.issued2014-04-01
dc.identifierpii: gku095
dc.identifier.citationWickramasinghe, V. O., Andrews, R., Ellis, P., Langford, C., Gurdon, J. B., Stewart, M., Venkitaraman, A. R. & Laskey, R. A. (2014). Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP. NUCLEIC ACIDS RESEARCH, 42 (8), pp.5059-5071. https://doi.org/10.1093/nar/gku095.
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/11343/257550
dc.description.abstractThe nuclear phase of the gene expression pathway culminates in the export of mature messenger RNAs (mRNAs) to the cytoplasm through nuclear pore complexes. GANP (germinal- centre associated nuclear protein) promotes the transfer of mRNAs bound to the transport factor NXF1 to nuclear pore complexes. Here, we demonstrate that GANP, subunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent transcripts were highly expressed, yet short-lived, and were highly enriched in those encoding central components of the gene expression machinery such as RNA synthesis and processing factors. After injection into Xenopus oocyte nuclei, representative GANP-dependent transcripts showed faster nuclear export kinetics than representative transcripts that were not influenced by GANP depletion. We propose that GANP promotes the nuclear export of specific classes of mRNAs that may facilitate rapid changes in gene expression.
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS
dc.titleSelective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP
dc.typeJournal Article
dc.identifier.doi10.1093/nar/gku095
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.source.titleNucleic Acids Research
melbourne.source.volume42
melbourne.source.issue8
melbourne.source.pages5059-5071
dc.rights.licenseCC BY
melbourne.elementsid1180028
melbourne.contributor.authorWickramasinghe, Vihandha
dc.identifier.eissn1362-4962
melbourne.accessrightsOpen Access


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