An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
AuthorHiga, Y; Uemura, R; Yamazaki, W; Goto, S; Goto, Y; Sueyoshi, M
Source TitleJournal of Veterinary Medical Science
PublisherJAPAN SOC VET SCI
University of Melbourne Author/sMasukagami, Yumiko
AffiliationSchool of BioSciences
Document TypeJournal Article
CitationsHiga, Y., Uemura, R., Yamazaki, W., Goto, S., Goto, Y. & Sueyoshi, M. (2016). An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis. JOURNAL OF VETERINARY MEDICAL SCIENCE, 78 (8), pp.1343-1346. https://doi.org/10.1292/jvms.15-0459.
Access StatusOpen Access
We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains. M. bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab samples were tested directly using our LAMP assays, the previously reported LAMP assay, conventional PCR assay without pre-culture and comparing standard culture methods. The improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively (with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was increased compared to existing methods.
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