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dc.contributor.authorHiga, Y
dc.contributor.authorUemura, R
dc.contributor.authorYamazaki, W
dc.contributor.authorGoto, S
dc.contributor.authorGoto, Y
dc.contributor.authorSueyoshi, M
dc.date.accessioned2020-12-21T04:17:54Z
dc.date.available2020-12-21T04:17:54Z
dc.date.issued2016-08-01
dc.identifier.citationHiga, Y., Uemura, R., Yamazaki, W., Goto, S., Goto, Y. & Sueyoshi, M. (2016). An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis. JOURNAL OF VETERINARY MEDICAL SCIENCE, 78 (8), pp.1343-1346. https://doi.org/10.1292/jvms.15-0459.
dc.identifier.issn0916-7250
dc.identifier.urihttp://hdl.handle.net/11343/257563
dc.description.abstractWe improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains. M. bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab samples were tested directly using our LAMP assays, the previously reported LAMP assay, conventional PCR assay without pre-culture and comparing standard culture methods. The improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively (with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was increased compared to existing methods.
dc.languageEnglish
dc.publisherJAPAN SOC VET SCI
dc.titleAn improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
dc.typeJournal Article
dc.identifier.doi10.1292/jvms.15-0459
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titleJournal of Veterinary Medical Science
melbourne.source.volume78
melbourne.source.issue8
melbourne.source.pages1343-1346
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1182941
melbourne.contributor.authorMasukagami, Yumiko
dc.identifier.eissn1347-7439
melbourne.accessrightsOpen Access


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