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dc.contributor.authorHoe, E
dc.contributor.authorNathanielsz, J
dc.contributor.authorToh, ZQ
dc.contributor.authorSpry, L
dc.contributor.authorMarimla, R
dc.contributor.authorBalloch, A
dc.contributor.authorMulholland, K
dc.contributor.authorLicciardi, PV
dc.date.accessioned2020-12-21T04:19:01Z
dc.date.available2020-12-21T04:19:01Z
dc.date.issued2016-12-01
dc.identifierpii: nu8120806
dc.identifier.citationHoe, E., Nathanielsz, J., Toh, Z. Q., Spry, L., Marimla, R., Balloch, A., Mulholland, K. & Licciardi, P. V. (2016). Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection. NUTRIENTS, 8 (12), https://doi.org/10.3390/nu8120806.
dc.identifier.issn2072-6643
dc.identifier.urihttp://hdl.handle.net/11343/257571
dc.description.abstractVitamin D induces a diverse range of biological effects, including important functions in bone health, calcium homeostasis and, more recently, on immune function. The role of vitamin D during infection is of particular interest given data from epidemiological studies suggesting that vitamin D deficiency is associated with an increased risk of infection. Vitamin D has diverse immunomodulatory functions, although its role during bacterial infection remains unclear. In this study, we examined the effects of 1,25(OH)₂D₃, the active metabolite of vitamin D, on peripheral blood mononuclear cells (PBMCs) and purified immune cell subsets isolated from healthy adults following stimulation with the bacterial ligands heat-killed pneumococcal serotype 19F (HK19F) and lipopolysaccharide (LPS). We found that 1,25(OH)₂D₃ significantly reduced pro-inflammatory cytokines TNF-α, IFN-γ, and IL-1β as well as the chemokine IL-8 for both ligands (three- to 53-fold), while anti-inflammatory IL-10 was increased (two-fold, p = 0.016) in HK19F-stimulated monocytes. Levels of HK19F-specific IFN-γ were significantly higher (11.7-fold, p = 0.038) in vitamin D-insufficient adults (<50 nmol/L) compared to sufficient adults (>50 nmol/L). Vitamin D also shifted the pro-inflammatory/anti-inflammatory balance towards an anti-inflammatory phenotype and increased the CD14 expression on monocytes (p = 0.008) in response to LPS but not HK19F stimulation. These results suggest that 1,25(OH)₂D₃ may be an important regulator of the inflammatory response and supports further in vivo and clinical studies to confirm the potential benefits of vitamin D in this context.
dc.languageEnglish
dc.publisherMDPI
dc.titleAnti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection
dc.typeJournal Article
dc.identifier.doi10.3390/nu8120806
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.source.titleNutrients
melbourne.source.volume8
melbourne.source.issue12
dc.rights.licenseCC BY
melbourne.elementsid1183257
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5188461
melbourne.contributor.authorMulholland, Edward
dc.identifier.eissn2072-6643
melbourne.accessrightsOpen Access


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