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dc.contributor.authorMiles, LB
dc.contributor.authorDarido, C
dc.contributor.authorKaslin, J
dc.contributor.authorHeath, JK
dc.contributor.authorJane, SM
dc.contributor.authorDworkin, S
dc.date.accessioned2020-12-21T04:26:26Z
dc.date.available2020-12-21T04:26:26Z
dc.date.issued2017-12-14
dc.identifierpii: 10.1038/s41598-017-17898-7
dc.identifier.citationMiles, L. B., Darido, C., Kaslin, J., Heath, J. K., Jane, S. M. & Dworkin, S. (2017). Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension. SCIENTIFIC REPORTS, 7 (1), https://doi.org/10.1038/s41598-017-17898-7.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/257625
dc.description.abstractThe grainyhead-like (grhl) transcription factors play crucial roles in craniofacial development, epithelial morphogenesis, neural tube closure, and dorso-ventral patterning. By utilising the zebrafish to differentially regulate expression of family members grhl2b and grhl3, we show that both genes regulate epithelial migration, particularly convergence-extension (CE) type movements, during embryogenesis. Genetic deletion of grhl3 via CRISPR/Cas9 results in failure to complete epiboly and pre-gastrulation embryonic rupture, whereas morpholino (MO)-mediated knockdown of grhl3 signalling leads to aberrant neural tube morphogenesis at the midbrain-hindbrain boundary (MHB), a phenotype likely due to a compromised overlying enveloping layer (EVL). Further disruptions of grhl3-dependent pathways (through co-knockdown of grhl3 with target genes spec1 and arhgef19) confirm significant MHB morphogenesis and neural tube closure defects. Concomitant MO-mediated disruption of both grhl2b and grhl3 results in further extensive CE-like defects in body patterning, notochord and somite morphogenesis. Interestingly, over-expression of either grhl2b or grhl3 also leads to numerous phenotypes consistent with disrupted cellular migration during gastrulation, including embryo dorsalisation, axial duplication and impaired neural tube migration leading to cyclopia. Taken together, our study ascribes novel roles to the Grhl family in the context of embryonic development and morphogenesis.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleMis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension
dc.typeJournal Article
dc.identifier.doi10.1038/s41598-017-17898-7
melbourne.affiliation.departmentSurgery (RMH)
melbourne.affiliation.departmentSir Peter MacCallum Department of Oncology
melbourne.source.titleScientific Reports
melbourne.source.volume7
melbourne.source.issue1
melbourne.identifier.nhmrc1106697
dc.rights.licenseCC BY
melbourne.elementsid1284168
melbourne.contributor.authorDarido, Charbel
melbourne.contributor.authorHeath, Joan
dc.identifier.eissn2045-2322
melbourne.identifier.fundernameidNHMRC, 1106697
melbourne.accessrightsOpen Access


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