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dc.contributor.authorScott, NE
dc.contributor.authorRogers, LD
dc.contributor.authorPrudova, A
dc.contributor.authorBrown, NF
dc.contributor.authorFortelny, N
dc.contributor.authorOverall, CM
dc.contributor.authorFoster, LJ
dc.date.accessioned2020-12-21T04:30:53Z
dc.date.available2020-12-21T04:30:53Z
dc.date.issued2017-01-01
dc.identifier.citationScott, N. E., Rogers, L. D., Prudova, A., Brown, N. F., Fortelny, N., Overall, C. M. & Foster, L. J. (2017). Interactome disassembly during apoptosis occurs independent of caspase cleavage. MOLECULAR SYSTEMS BIOLOGY, 13 (1), https://doi.org/10.15252/msb.20167067.
dc.identifier.issn1744-4292
dc.identifier.urihttp://hdl.handle.net/11343/257655
dc.description.abstractProtein-protein interaction networks (interactomes) define the functionality of all biological systems. In apoptosis, proteolysis by caspases is thought to initiate disassembly of protein complexes and cell death. Here we used a quantitative proteomics approach, protein correlation profiling (PCP), to explore changes in cytoplasmic and mitochondrial interactomes in response to apoptosis initiation as a function of caspase activity. We measured the response to initiation of Fas-mediated apoptosis in 17,991 interactions among 2,779 proteins, comprising the largest dynamic interactome to date. The majority of interactions were unaffected early in apoptosis, but multiple complexes containing known caspase targets were disassembled. Nonetheless, proteome-wide analysis of proteolytic processing by terminal amine isotopic labeling of substrates (TAILS) revealed little correlation between proteolytic and interactome changes. Our findings show that, in apoptosis, significant interactome alterations occur before and independently of caspase activity. Thus, apoptosis initiation includes a tight program of interactome rearrangement, leading to disassembly of relatively few, select complexes. These early interactome alterations occur independently of cleavage of these protein by caspases.
dc.languageEnglish
dc.publisherWILEY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleInteractome disassembly during apoptosis occurs independent of caspase cleavage
dc.typeJournal Article
dc.identifier.doi10.15252/msb.20167067
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleMolecular Systems Biology
melbourne.source.volume13
melbourne.source.issue1
melbourne.identifier.nhmrc1037373
dc.rights.licenseCC BY
melbourne.elementsid1286147
melbourne.contributor.authorScott, Nichollas
dc.identifier.eissn1744-4292
melbourne.identifier.fundernameidNHMRC, 1037373
melbourne.accessrightsOpen Access


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