University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • University Services
  • University General - Research Publications
  • View Item
  • Minerva Access
  • University Services
  • University General - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

    Thumbnail
    Download
    Published version (1.662Mb)

    Citations
    Scopus
    Web of Science
    Altmetric
    18
    16
    Author
    Quek, C; Bellingham, SA; Jung, C-H; Scicluna, BJ; Shambrook, MC; Sharples, RA; Cheng, L; Hill, AF
    Date
    2017-01-01
    Source Title
    RNA Biology
    Publisher
    TAYLOR & FRANCIS INC
    University of Melbourne Author/s
    SHAMBROOK, MITCH; Hill, Andrew; Bellingham, Shayne; Jung, Chol-Hee; QUEK, CAMELIA; Cheng, Lesley
    Affiliation
    University General
    Biochemistry and Molecular Biology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Quek, C., Bellingham, S. A., Jung, C. -H., Scicluna, B. J., Shambrook, M. C., Sharples, R. A., Cheng, L. & Hill, A. F. (2017). Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery. RNA BIOLOGY, 14 (2), pp.245-258. https://doi.org/10.1080/15476286.2016.1270005.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257676
    DOI
    10.1080/15476286.2016.1270005
    Abstract
    Small non-coding RNAs (ncRNA), including microRNAs (miRNA), enclosed in exosomes are being utilised for biomarker discovery in disease. Two common exosome isolation methods involve differential ultracentrifugation or differential ultracentrifugation coupled with Optiprep gradient fractionation. Generally, the incorporation of an Optiprep gradient provides better separation and increased purity of exosomes. The question of whether increased purity of exosomes is required for small ncRNA profiling, particularly in diagnostic and biomarker purposes, has not been addressed and highly debated. Utilizing an established neuronal cell system, we used next-generation sequencing to comprehensively profile ncRNA in cells and exosomes isolated by these 2 isolation methods. By comparing ncRNA content in exosomes from these two methods, we found that exosomes from both isolation methods were enriched with miRNAs and contained a diverse range of rRNA, small nuclear RNA, small nucleolar RNA and piwi-interacting RNA as compared with their cellular counterparts. Additionally, tRNA fragments (30-55 nucleotides in length) were identified in exosomes and may act as potential modulators for repressing protein translation. Overall, the outcome of this study confirms that ultracentrifugation-based method as a feasible approach to identify ncRNA biomarkers in exosomes.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [53102]
    • Biochemistry and Molecular Biology - Research Publications [1075]
    • University General - Research Publications [467]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors