Show simple item record

dc.contributor.authorQuek, C
dc.contributor.authorBellingham, SA
dc.contributor.authorJung, C-H
dc.contributor.authorScicluna, BJ
dc.contributor.authorShambrook, MC
dc.contributor.authorSharples, RA
dc.contributor.authorCheng, L
dc.contributor.authorHill, AF
dc.date.accessioned2020-12-22T02:37:45Z
dc.date.available2020-12-22T02:37:45Z
dc.date.issued2017-01-01
dc.identifier.citationQuek, C., Bellingham, S. A., Jung, C. -H., Scicluna, B. J., Shambrook, M. C., Sharples, R. A., Cheng, L. & Hill, A. F. (2017). Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery. RNA BIOLOGY, 14 (2), pp.245-258. https://doi.org/10.1080/15476286.2016.1270005.
dc.identifier.issn1547-6286
dc.identifier.urihttp://hdl.handle.net/11343/257676
dc.description.abstractSmall non-coding RNAs (ncRNA), including microRNAs (miRNA), enclosed in exosomes are being utilised for biomarker discovery in disease. Two common exosome isolation methods involve differential ultracentrifugation or differential ultracentrifugation coupled with Optiprep gradient fractionation. Generally, the incorporation of an Optiprep gradient provides better separation and increased purity of exosomes. The question of whether increased purity of exosomes is required for small ncRNA profiling, particularly in diagnostic and biomarker purposes, has not been addressed and highly debated. Utilizing an established neuronal cell system, we used next-generation sequencing to comprehensively profile ncRNA in cells and exosomes isolated by these 2 isolation methods. By comparing ncRNA content in exosomes from these two methods, we found that exosomes from both isolation methods were enriched with miRNAs and contained a diverse range of rRNA, small nuclear RNA, small nucleolar RNA and piwi-interacting RNA as compared with their cellular counterparts. Additionally, tRNA fragments (30-55 nucleotides in length) were identified in exosomes and may act as potential modulators for repressing protein translation. Overall, the outcome of this study confirms that ultracentrifugation-based method as a feasible approach to identify ncRNA biomarkers in exosomes.
dc.languageEnglish
dc.publisherTAYLOR & FRANCIS INC
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0
dc.titleDefining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery
dc.typeJournal Article
dc.identifier.doi10.1080/15476286.2016.1270005
melbourne.affiliation.departmentUniversity General
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.source.titleRNA Biology
melbourne.source.volume14
melbourne.source.issue2
melbourne.source.pages245-258
dc.rights.licenseCC BY-NC
melbourne.elementsid1122958
melbourne.contributor.authorSHAMBROOK, MITCH
melbourne.contributor.authorHill, Andrew
melbourne.contributor.authorBellingham, Shayne
melbourne.contributor.authorJung, Chol-Hee
melbourne.contributor.authorQUEK, CAMELIA
melbourne.contributor.authorCheng, Lesley
dc.identifier.eissn1555-8584
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record