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dc.contributor.authorVoskamp, AL
dc.contributor.authorPrickett, SR
dc.contributor.authorMackay, F
dc.contributor.authorRolland, JM
dc.contributor.authorO'Hehir, RE
dc.date.accessioned2020-12-22T02:44:15Z
dc.date.available2020-12-22T02:44:15Z
dc.date.issued2013-12-09
dc.identifierpii: PONE-D-13-35393
dc.identifier.citationVoskamp, A. L., Prickett, S. R., Mackay, F., Rolland, J. M. & O'Hehir, R. E. (2013). MHC Class II Expression in Human Basophils: Induction and Lack of Functional Significance. PLOS ONE, 8 (12), https://doi.org/10.1371/journal.pone.0081777.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/257701
dc.description.abstractThe antigen-presenting abilities of basophils and their role in initiating a Th2 phenotype is a topic of current controversy. We aimed to determine whether human basophils can be induced to express MHC Class II and act as antigen presenting cells for T cell stimulation. Isolated human basophils were exposed to a panel of cytokines and TLR-ligands and assessed for MHC Class II expression. MHC Class II was expressed in up to 17% of isolated basophils following incubation with a combination of IL-3, IFN-γ and GM-CSF for 72 hours. Costimulatory molecules (CD80 and CD86) were expressed at very low levels after stimulation. Gene expression analysis of MHC Class II-positive basophils confirmed up-regulation of HLA-DR, HLA-DM, CD74 and Cathepsin S. However, MHC Class II expressing basophils were incapable of inducing antigen-specific T cell activation or proliferation. This is the first report of significant cytokine-induced MHC Class II up-regulation, at both RNA and protein level, in isolated human basophils. By testing stimulation with relevant T cell epitope peptide as well as whole antigen, the failure of MHC Class II expressing basophils to induce T cell response was shown not to be solely due to inefficient antigen uptake and/or processing.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleMHC Class II Expression in Human Basophils: Induction and Lack of Functional Significance
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0081777
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titlePLoS One
melbourne.source.volume8
melbourne.source.issue12
dc.rights.licenseCC BY
melbourne.elementsid999443
melbourne.contributor.authorMackay, Fabienne
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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