dc.contributor.author | Voskamp, AL | |
dc.contributor.author | Prickett, SR | |
dc.contributor.author | Mackay, F | |
dc.contributor.author | Rolland, JM | |
dc.contributor.author | O'Hehir, RE | |
dc.date.accessioned | 2020-12-22T02:44:15Z | |
dc.date.available | 2020-12-22T02:44:15Z | |
dc.date.issued | 2013-12-09 | |
dc.identifier | pii: PONE-D-13-35393 | |
dc.identifier.citation | Voskamp, A. L., Prickett, S. R., Mackay, F., Rolland, J. M. & O'Hehir, R. E. (2013). MHC Class II Expression in Human Basophils: Induction and Lack of Functional Significance. PLOS ONE, 8 (12), https://doi.org/10.1371/journal.pone.0081777. | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/11343/257701 | |
dc.description.abstract | The antigen-presenting abilities of basophils and their role in initiating a Th2 phenotype is a topic of current controversy. We aimed to determine whether human basophils can be induced to express MHC Class II and act as antigen presenting cells for T cell stimulation. Isolated human basophils were exposed to a panel of cytokines and TLR-ligands and assessed for MHC Class II expression. MHC Class II was expressed in up to 17% of isolated basophils following incubation with a combination of IL-3, IFN-γ and GM-CSF for 72 hours. Costimulatory molecules (CD80 and CD86) were expressed at very low levels after stimulation. Gene expression analysis of MHC Class II-positive basophils confirmed up-regulation of HLA-DR, HLA-DM, CD74 and Cathepsin S. However, MHC Class II expressing basophils were incapable of inducing antigen-specific T cell activation or proliferation. This is the first report of significant cytokine-induced MHC Class II up-regulation, at both RNA and protein level, in isolated human basophils. By testing stimulation with relevant T cell epitope peptide as well as whole antigen, the failure of MHC Class II expressing basophils to induce T cell response was shown not to be solely due to inefficient antigen uptake and/or processing. | |
dc.language | English | |
dc.publisher | PUBLIC LIBRARY SCIENCE | |
dc.title | MHC Class II Expression in Human Basophils: Induction and Lack of Functional Significance | |
dc.type | Journal Article | |
dc.identifier.doi | 10.1371/journal.pone.0081777 | |
melbourne.affiliation.department | Microbiology and Immunology | |
melbourne.source.title | PLoS One | |
melbourne.source.volume | 8 | |
melbourne.source.issue | 12 | |
dc.rights.license | CC BY | |
melbourne.elementsid | 999443 | |
melbourne.contributor.author | Mackay, Fabienne | |
dc.identifier.eissn | 1932-6203 | |
melbourne.accessrights | Open Access | |