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dc.contributor.authorFord, KL
dc.contributor.authorZeng, W
dc.contributor.authorHeazlewood, JL
dc.contributor.authorBacic, A
dc.date.accessioned2020-12-22T02:46:49Z
dc.date.available2020-12-22T02:46:49Z
dc.date.issued2015-08-28
dc.identifier.citationFord, K. L., Zeng, W., Heazlewood, J. L. & Bacic, A. (2015). Characterization of protein N-glycosylation by tandem mass spectrometry using complementary fragmentation techniques. FRONTIERS IN PLANT SCIENCE, 6 (AUG), https://doi.org/10.3389/fpls.2015.00674.
dc.identifier.issn1664-462X
dc.identifier.urihttp://hdl.handle.net/11343/257710
dc.description.abstractThe analysis of post-translational modifications (PTMs) by proteomics is regarded as a technically challenging undertaking. While in recent years approaches to examine and quantify protein phosphorylation have greatly improved, the analysis of many protein modifications, such as glycosylation, are still regarded as problematic. Limitations in the standard proteomics workflow, such as use of suboptimal peptide fragmentation methods, can significantly prevent the identification of glycopeptides. The current generation of tandem mass spectrometers has made available a variety of fragmentation options, many of which are becoming standard features on these instruments. We have used three common fragmentation techniques, namely CID, HCD, and ETD, to analyze a glycopeptide and highlight how an integrated fragmentation approach can be used to identify the modified residue and characterize the N-glycan on a peptide.
dc.languageEnglish
dc.publisherFRONTIERS MEDIA SA
dc.titleCharacterization of protein N-glycosylation by tandem mass spectrometry using complementary fragmentation techniques
dc.typeJournal Article
dc.identifier.doi10.3389/fpls.2015.00674
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titleFrontiers in Plant Science
melbourne.source.volume6
melbourne.source.issueAUG
dc.rights.licenseCC BY
melbourne.elementsid1002425
melbourne.contributor.authorHeazlewood, Joshua
melbourne.contributor.authorBacic, Anthony
melbourne.contributor.authorFord, Kristina
melbourne.contributor.authorZeng, Wei
dc.identifier.eissn1664-462X
melbourne.identifier.fundernameidUNIVERSITY OF MELBOURNE, CE110001007
melbourne.identifier.fundernameidUNIVERSITY OF ADELAIDE, CE110001007
melbourne.accessrightsOpen Access


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