Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey
AuthorGardiner, C; Di Vizio, D; Sahoo, S; Thery, C; Witwer, KW; Wauben, M; Hill, AF
Source TitleJournal of Extracellular Vesicles
PublisherTAYLOR & FRANCIS LTD
University of Melbourne Author/sHill, Andrew
AffiliationBiochemistry and Molecular Biology
Document TypeJournal Article
CitationsGardiner, C., Di Vizio, D., Sahoo, S., Thery, C., Witwer, K. W., Wauben, M. & Hill, A. F. (2016). Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. JOURNAL OF EXTRACELLULAR VESICLES, 5 (1), https://doi.org/10.3402/jev.v5.32945.
Access StatusOpen Access
Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype between different populations of EVs. Little is known about current practices for the isolation, purification and characterization of EVs. We report here the first large, detailed survey of current worldwide practices for the isolation and characterization of EVs. Conditioned cell culture media was the most widely used material (83%). Ultracentrifugation remains the most commonly used isolation method (81%) with 59% of respondents use a combination of methods. Only 9% of respondents used only 1 characterization method, with others using 2 or more methods. Sample volume, sample type and downstream application all influenced the isolation and characterization techniques employed.
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References