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    Real-time qPCR improves meningitis pathogen detection in invasive bacterial-vaccine preventable disease surveillance in Fiji

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    Author
    Dunne, EM; Mantanitobua, S; Singh, SP; Reyburn, R; Tuivaga, E; Rafai, E; Tikoduadua, L; Porter, B; Satzke, C; Strachan, JE; ...
    Date
    2016-12-23
    Source Title
    Scientific Reports
    Publisher
    NATURE PUBLISHING GROUP
    University of Melbourne Author/s
    Russell, Fiona; Satzke, Catherine; Strachan, Janet; Jenney, Adam
    Affiliation
    Paediatrics (RCH)
    Microbiology and Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Dunne, E. M., Mantanitobua, S., Singh, S. P., Reyburn, R., Tuivaga, E., Rafai, E., Tikoduadua, L., Porter, B., Satzke, C., Strachan, J. E., Fox, K. K., Jenkins, K. M., Jenney, A., Baro, S., Mulholland, E. K., Kama, M. & Russell, F. M. (2016). Real-time qPCR improves meningitis pathogen detection in invasive bacterial-vaccine preventable disease surveillance in Fiji. SCIENTIFIC REPORTS, 6 (1), https://doi.org/10.1038/srep39784.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257846
    DOI
    10.1038/srep39784
    Abstract
    As part of the World Health Organization Invasive Bacterial-Vaccine Preventable Diseases (IB-VPD) surveillance in Suva, Fiji, cerebrospinal fluid (CSF) samples from suspected meningitis patients of all ages were examined by traditional methods (culture, Gram stain, and latex agglutination for bacterial antigen) and qPCR for Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae. Of 266 samples tested, pathogens were identified in 47 (17.7%). S. pneumoniae was the most common pathogen detected (n = 17) followed by N. meningitidis (n = 13). The use of qPCR significantly increased detection of IB-VPD pathogens (P = 0.0001): of 35 samples that were qPCR positive for S. pneumoniae, N. meningitidis, and H. influenzae, only 10 were culture positive. This was particularly relevant for N. meningitidis, as only 1/13 cases was culture positive. Molecular serotyping by microarray was used to determine pneumococcal serotypes from 9 of 16 (56%) of samples using DNA directly extracted from CSF specimens. Results indicate that qPCR significantly increases detection of S. pneumoniae, N. meningitidis, and H. influenzae in CSF, and that application of molecular diagnostics is a feasible way to enhance local and global surveillance for IB-VPD.

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