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dc.contributor.authorCifuentes, M
dc.contributor.authorRivard, M
dc.contributor.authorPereira, L
dc.contributor.authorChelysheva, L
dc.contributor.authorMercier, R
dc.date.accessioned2020-12-22T03:27:41Z
dc.date.available2020-12-22T03:27:41Z
dc.date.issued2013-08-07
dc.identifierpii: PONE-D-13-16469
dc.identifier.citationCifuentes, M., Rivard, M., Pereira, L., Chelysheva, L. & Mercier, R. (2013). Haploid Meiosis in Arabidopsis: Double-Strand Breaks Are Formed and Repaired but Without Synapsis and Crossovers. PLOS ONE, 8 (8), https://doi.org/10.1371/journal.pone.0072431.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/257858
dc.description.abstractTwo hallmark features of meiosis are i) the formation of crossovers (COs) between homologs and ii) the production of genetically-unique haploid spores that will fuse to restore the somatic ploidy level upon fertilization. In this study we analysed meiosis in haploid Arabidopsis thaliana plants and a range of haploid mutants to understand how meiosis progresses without a homolog. Extremely low chiasma frequency and very limited synapsis occurred in wild-type haploids. The resulting univalents segregated in two uneven groups at the first division, and sister chromatids segregated to opposite poles at the second division, leading to the production of unbalanced spores. DNA double-strand breaks that initiate meiotic recombination were formed, but in half the number compared to diploid meiosis. They were repaired in a RAD51- and REC8-dependent manner, but independently of DMC1, presumably using the sister chromatid as a template. Additionally, turning meiosis into mitosis (MiMe genotype) in haploids resulted in the production of balanced haploid gametes and restoration of fertility. The variability of the effect on meiosis of the absence of homologous chromosomes in different organisms is then discussed.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleHaploid Meiosis in Arabidopsis: Double-Strand Breaks Are Formed and Repaired but Without Synapsis and Crossovers
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0072431
melbourne.affiliation.departmentAgriculture and Food Systems
melbourne.source.titlePLoS One
melbourne.source.volume8
melbourne.source.issue8
dc.rights.licenseCC BY
melbourne.elementsid1174180
melbourne.contributor.authorCifuentes, Marta
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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