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dc.contributor.authorJani, D
dc.contributor.authorLutz, S
dc.contributor.authorHurt, E
dc.contributor.authorLaskey, RA
dc.contributor.authorStewart, M
dc.contributor.authorWickramasinghe, VO
dc.date.accessioned2020-12-22T03:32:44Z
dc.date.available2020-12-22T03:32:44Z
dc.date.issued2012-05-01
dc.identifierpii: gks059
dc.identifier.citationJani, D., Lutz, S., Hurt, E., Laskey, R. A., Stewart, M. & Wickramasinghe, V. O. (2012). Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export. NUCLEIC ACIDS RESEARCH, 40 (10), pp.4562-4573. https://doi.org/10.1093/nar/gks059.
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/11343/257876
dc.description.abstractExport of messenger RNA (mRNA) from the nucleus to the cytoplasm is a critical step in the gene expression pathway of eukaryotic cells. Here, we report the functional and structural characterization of the mammalian TREX-2 complex and show how it links transcription/processing with nuclear mRNA export. Mammalian TREX-2 is based on a germinal-centre associated nuclear protein (GANP) scaffold to which ENY2, PCID2 and centrins bind and depletion of any of these components inhibits mRNA export. The crystal structure of the GANP:ENY2 complex shows that two ENY2 chains interact directly with GANP, but they have different orientations from those observed on yeast Sac3. GANP is required to recruit ENY2 to nuclear pore complexes (NPCs), but ENY2 is not necessary to recruit GANP, which requires both its CID and MCM3AP domains, together with nucleoporin Nup153. GANP and ENY2 associate with RNA polymerase II and inhibition of mRNA processing redistributes GANP from NPCs into nuclear foci indicating that mammalian TREX-2 is associated with transcription. Thus, we implicate TREX-2 as an integral component of the mammalian mRNA export machinery where it links transcription and nuclear export by facilitating the transfer of mature mRNPs from the nuclear interior to NPCs.
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0
dc.titleFunctional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export
dc.typeJournal Article
dc.identifier.doi10.1093/nar/gks059
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.source.titleNucleic Acids Research
melbourne.source.volume40
melbourne.source.issue10
melbourne.source.pages4562-4573
dc.rights.licenseCC BY-NC
melbourne.elementsid1180027
melbourne.contributor.authorWickramasinghe, Vihandha
dc.identifier.eissn1362-4962
melbourne.accessrightsOpen Access


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