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    SCA-1 Labels a Subset of Estrogen-Responsive Bipotential Repopulating Cells within the CD24(+) CD49f(hi) Mammary Stem Cell-Enriched Compartment

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    Author
    Dall, GV; Vieusseux, JL; Korach, KS; Arao, Y; Hewitt, SC; Hamilton, KJ; Dzierzak, E; Boon, WC; Simpson, ER; Ramsay, RG; ...
    Date
    2017-02-14
    Source Title
    Stem Cell Reports
    Publisher
    CELL PRESS
    University of Melbourne Author/s
    Anderson, Robin; Ramsay, Robert; Britt, Kara; Boon, Wah Chin; Risbridger, Gail
    Affiliation
    Sir Peter MacCallum Department of Oncology
    School of BioSciences
    Metadata
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    Document Type
    Journal Article
    Citations
    Dall, G. V., Vieusseux, J. L., Korach, K. S., Arao, Y., Hewitt, S. C., Hamilton, K. J., Dzierzak, E., Boon, W. C., Simpson, E. R., Ramsay, R. G., Stein, T., Morris, J. S., Anderson, R. L., Risbridger, G. P. & Britt, K. L. (2017). SCA-1 Labels a Subset of Estrogen-Responsive Bipotential Repopulating Cells within the CD24(+) CD49f(hi) Mammary Stem Cell-Enriched Compartment. STEM CELL REPORTS, 8 (2), pp.417-431. https://doi.org/10.1016/j.stemcr.2016.12.022.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257877
    DOI
    10.1016/j.stemcr.2016.12.022
    Abstract
    Estrogen stimulates breast development during puberty and mammary tumors in adulthood through estrogen receptor-α (ERα). These effects are proposed to occur via ERα+ luminal cells and not the mammary stem cells (MaSCs) that are ERαneg. Since ERα+ luminal cells express stem cell antigen-1 (SCA-1), we sought to determine if SCA-1 could define an ERα+ subset of EpCAM+/CD24+/CD49fhi MaSCs. We show that the MaSC population has a distinct SCA-1+ population that is abundant in pre-pubertal mammary glands. The SCA-1+ MaSCs have less stem cell markers and less in vivo repopulating activity than their SCA-1neg counterparts. However, they express ERα and specifically enter the cell cycle at puberty. Using estrogen-deficient aromatase knockouts (ArKO), we showed that the SCA-1+ MaSC could be directly modulated by estrogen supplementation. Thus, SCA-1 enriches for an ERα+, estrogen-sensitive subpopulation within the CD24+/CD49fhi MaSC population that may be responsible for the hormonal sensitivity of the developing mammary gland.

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