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dc.contributor.authorDall, GV
dc.contributor.authorVieusseux, JL
dc.contributor.authorKorach, KS
dc.contributor.authorArao, Y
dc.contributor.authorHewitt, SC
dc.contributor.authorHamilton, KJ
dc.contributor.authorDzierzak, E
dc.contributor.authorBoon, WC
dc.contributor.authorSimpson, ER
dc.contributor.authorRamsay, RG
dc.contributor.authorStein, T
dc.contributor.authorMorris, JS
dc.contributor.authorAnderson, RL
dc.contributor.authorRisbridger, GP
dc.contributor.authorBritt, KL
dc.date.accessioned2020-12-22T03:33:03Z
dc.date.available2020-12-22T03:33:03Z
dc.date.issued2017-02-14
dc.identifierpii: S2213-6711(16)30312-5
dc.identifier.citationDall, G. V., Vieusseux, J. L., Korach, K. S., Arao, Y., Hewitt, S. C., Hamilton, K. J., Dzierzak, E., Boon, W. C., Simpson, E. R., Ramsay, R. G., Stein, T., Morris, J. S., Anderson, R. L., Risbridger, G. P. & Britt, K. L. (2017). SCA-1 Labels a Subset of Estrogen-Responsive Bipotential Repopulating Cells within the CD24(+) CD49f(hi) Mammary Stem Cell-Enriched Compartment. STEM CELL REPORTS, 8 (2), pp.417-431. https://doi.org/10.1016/j.stemcr.2016.12.022.
dc.identifier.issn2213-6711
dc.identifier.urihttp://hdl.handle.net/11343/257877
dc.description.abstractEstrogen stimulates breast development during puberty and mammary tumors in adulthood through estrogen receptor-α (ERα). These effects are proposed to occur via ERα+ luminal cells and not the mammary stem cells (MaSCs) that are ERαneg. Since ERα+ luminal cells express stem cell antigen-1 (SCA-1), we sought to determine if SCA-1 could define an ERα+ subset of EpCAM+/CD24+/CD49fhi MaSCs. We show that the MaSC population has a distinct SCA-1+ population that is abundant in pre-pubertal mammary glands. The SCA-1+ MaSCs have less stem cell markers and less in vivo repopulating activity than their SCA-1neg counterparts. However, they express ERα and specifically enter the cell cycle at puberty. Using estrogen-deficient aromatase knockouts (ArKO), we showed that the SCA-1+ MaSC could be directly modulated by estrogen supplementation. Thus, SCA-1 enriches for an ERα+, estrogen-sensitive subpopulation within the CD24+/CD49fhi MaSC population that may be responsible for the hormonal sensitivity of the developing mammary gland.
dc.languageEnglish
dc.publisherCELL PRESS
dc.titleSCA-1 Labels a Subset of Estrogen-Responsive Bipotential Repopulating Cells within the CD24(+) CD49f(hi) Mammary Stem Cell-Enriched Compartment
dc.typeJournal Article
dc.identifier.doi10.1016/j.stemcr.2016.12.022
melbourne.affiliation.departmentSir Peter MacCallum Department of Oncology
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titleStem Cell Reports
melbourne.source.volume8
melbourne.source.issue2
melbourne.source.pages417-431
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1180056
melbourne.contributor.authorAnderson, Robin
melbourne.contributor.authorRamsay, Robert
melbourne.contributor.authorBritt, Kara
melbourne.contributor.authorBoon, Wah Chin
melbourne.contributor.authorRisbridger, Gail
dc.identifier.eissn2213-6711
melbourne.accessrightsOpen Access


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