University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Affiliates
  • Centre for Eye Research Australia (CERA)
  • Centre for Eye Research Australia (CERA) - Research Publications
  • View Item
  • Minerva Access
  • Affiliates
  • Centre for Eye Research Australia (CERA)
  • Centre for Eye Research Australia (CERA) - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model

    Thumbnail
    Download
    Published version (5.131Mb)

    Citations
    Scopus
    Web of Science
    Altmetric
    31
    14
    Author
    Wang, L; Lee, AYW; Wigg, JP; Peshavariya, H; Liu, P; Zhang, H
    Date
    2016-06-01
    Source Title
    International Journal of Molecular Sciences
    Publisher
    MDPI
    University of Melbourne Author/s
    ZHANG, HONG; Peshavariya, Hitesh
    Affiliation
    Centre for Eye Research Australia (CERA)
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Wang, L., Lee, A. Y. W., Wigg, J. P., Peshavariya, H., Liu, P. & Zhang, H. (2016). miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 17 (6), https://doi.org/10.3390/ijms17060895.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/257981
    DOI
    10.3390/ijms17060895
    Abstract
    miR-126 has recently been implicated in modulating angiogenic factors in vascular development. Understandings its biological significance might enable development of therapeutic interventions for diseases like age-related macular degeneration (AMD). We aimed to determine the role of miR-126 in AMD using a laser-induced choroidal neovascularization (CNV) mouse model. CNV was induced by laser photocoagulation in C57BL/6 mice. The CNV mice were transfected with scrambled miR or miR-126 mimic. The expression of miR-126, vascular endothelial growth factor-A (VEGF-A), Kinase insert domain receptor (KDR) and Sprouty-related EVH1 domain-containing protein 1 (SPRED-1) in ocular tissues were analyzed by qPCR and Western blot. The overexpression effects of miR-126 were also proven on human microvascular endothelial cells (HMECs). miR-126 showed a significant decrease in CNV mice (p < 0.05). Both mRNA and protein levels of VEGF-A, KDR and SPRED-1 were upregulated with CNV; these changes were ameliorated by restoration of miR-126 (p < 0.05). CNV was reduced after miR-126 transfection. Transfection of miR-126 reduced the HMECs 2D-capillary-like tube formation (p < 0.01) and migration (p < 0.01). miR-126 has been shown to be a negative modulator of angiogenesis in the eye. All together these results high lights the therapeutic potential of miR-126 suggests that it may contribute as a putative therapeutic target for AMD in humans.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [53102]
    • Centre for Eye Research Australia (CERA) - Research Publications [103]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors