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dc.contributor.authorDhaliwal, I
dc.contributor.authorMason, AS
dc.contributor.authorBanga, S
dc.contributor.authorBharti, S
dc.contributor.authorKaur, B
dc.contributor.authorGurung, AM
dc.contributor.authorSalisbury, PA
dc.contributor.authorBatley, J
dc.contributor.authorBanga, SS
dc.date.accessioned2020-12-22T04:07:33Z
dc.date.available2020-12-22T04:07:33Z
dc.date.issued2017-01-01
dc.identifierpii: g3.116.036442
dc.identifier.citationDhaliwal, I., Mason, A. S., Banga, S., Bharti, S., Kaur, B., Gurung, A. M., Salisbury, P. A., Batley, J. & Banga, S. S. (2017). Cytogenetic and Molecular Characterization of B-Genome Introgression Lines of Brassica napus L.. G3-GENES GENOMES GENETICS, 7 (1), pp.77-86. https://doi.org/10.1534/g3.116.036442.
dc.identifier.issn2160-1836
dc.identifier.urihttp://hdl.handle.net/11343/258000
dc.description.abstractBrassica napus introgression lines (ILs), having B-genome segments from B. carinata, were assessed genetically for extent of introgression and phenotypically for siliqua shatter resistance. Introgression lines had 7-9% higher DNA content, were meiotically stable, and had almost normal pollen fertility/seed set. Segment introgressions were confirmed by fluorescent genomic in situ hybridization (fl-GISH), SSR analyses, and SNP studies. Genotyping with 48 B-genome specific SSRs detected substitutions from B3, B4, B6, and B7 chromosomes on 39 of the 69 ILs whereas SNP genotyping detected a total of 23 B-segments (≥3 Mb) from B4, B6, and B7 introgressed into 10 of the 19 (C1, C2, C3, C5, C6, C8, C9, A3, A9, A10) chromosomes in 17 ILs. The size of substitutions varied from 3.0 Mb on chromosome A9 (IL59) to 42.44 Mb on chromosome C2 (IL54), ranging from 7 to 83% of the recipient chromosome. Average siliqua strength in ILs was observed to be higher than that of B. napus parents (2.2-6.0 vs. 1.9-4.0 mJ) while siliqua strength in some of the lines was almost equal to that of the donor parent B. carinata (6.0 vs.7.2 mJ). These ILs, with large chunks of substituted B-genome, can prove to be a useful prebreeding resource for germplasm enhancement in B. napus, especially for siliqua shatter resistance.
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS INC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleCytogenetic and Molecular Characterization of B-Genome Introgression Lines of Brassica napus L.
dc.typeJournal Article
dc.identifier.doi10.1534/g3.116.036442
melbourne.affiliation.departmentAgriculture and Food Systems
melbourne.source.titleG3: Genes, Genomes, Genetics
melbourne.source.volume7
melbourne.source.issue1
melbourne.source.pages77-86
dc.rights.licenseCC BY
melbourne.elementsid1183545
melbourne.contributor.authorGurung, Allison
melbourne.contributor.authorSalisbury, Phillip
dc.identifier.eissn2160-1836
melbourne.accessrightsOpen Access


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