University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Ophthalmology (Eye & Ear Hospital)
  • Ophthalmology (Eye & Ear Hospital) - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Ophthalmology (Eye & Ear Hospital)
  • Ophthalmology (Eye & Ear Hospital) - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

    Thumbnail
    Download
    Published version (19.36Mb)

    Citations
    Scopus
    Web of Science
    Altmetric
    13
    10
    Author
    Mok, PL; Leow, SN; Koh, AE-H; Nizam, HHM; Ding, SLS; Luu, C; Ruhaslizan, R; Wong, HS; Halim, WHWA; Ng, MH; ...
    Date
    2017-02-01
    Source Title
    International Journal of Molecular Sciences
    Publisher
    MDPI
    University of Melbourne Author/s
    Luu, Chi; Luu, Chi
    Affiliation
    Ophthalmology (Eye & Ear Hospital)
    Centre for Eye Research Australia (CERA)
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Mok, P. L., Leow, S. N., Koh, A. E. -H., Nizam, H. H. M., Ding, S. L. S., Luu, C., Ruhaslizan, R., Wong, H. S., Halim, W. H. W. A., Ng, M. H., Idrus, R. B. H., Chowdhury, S. R., Bastion, C. M. -L., Subbiah, S. K., Higuchi, A., Alarfaj, A. A. & Then, K. Y. (2017). Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18 (2), https://doi.org/10.3390/ijms18020345.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/258005
    DOI
    10.3390/ijms18020345
    Abstract
    Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [53102]
    • Centre for Eye Research Australia (CERA) - Research Publications [103]
    • Ophthalmology (Eye & Ear Hospital) - Research Publications [599]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors